Supercritical Carbon Dioxide Extraction Of Puerarin And Pharmacodynamics Of Puerarin Extractive

ObjectiveChoose extraction of puerarin in radix puerariae best extraction processconditions and purification process conditions; Investigation of extracts fromradix puerariae chain with urea bacteria element （STZ） of rats caused by thediabetes nephrosis kidney damage of protection.MethodsIn reference to the literature, and on the basis of the supercritical CO₂fluidextraction in the extraction of puerarin puerarin,crushing the grain size forcertain functional materials, vacuum drying after extraction, concentrated crudeextractings get. According to the above process of first respectively extractionprocess of entrainment agents （alcohol） density, pressure, temperature, time forsingle factor experiment, to determine the effect of various factors, using theorthogonal design method to determine the best combination of extractionconditions, each factor take three level, the choice then L9（34） orthogonal tablearrangement experiment. And take water extraction, ultrasonic extractionmethod respectively puerarin extraction, with the puerarin consociation sibelinecontent as index, more three extraction methods of the advantages anddisadvantages, and seek the puerarin consociation sibeline cure the bestextraction method.With the content and the puerarin consociation sibeline cure rate as an extract of indexes, the crude extractings separation and purification, wereused respectively to alcohol sink method, natural sinking method，and highspeed centrifugation, through comparison method, find the best purificationmethods.In reference to the literature,and on the basis of urea in chain with bacteriaelement （STZ） of diabetic nephropathy as the animal diseases model, rats bloodsugar, kidney hypertrophy index,24h urine protein and kidney damagepathological form changes as the index to evaluate the efficacy of puerarinextract.ResultsIn supercritical CO₂extraction, water extraction, ultrasonic extraction threemethods to extract the puerarin consociation sibeline cure content differentresults, choose supercritical CO₂extraction. According to the analysis and theresults of variance analysis intuitive, influence the results of the extraction in the4factors, entrainment agents density of puerarin extraction effect is the biggest,is the main influencing factors; Next is the extraction pressure and extractiontime, the influence of the temperature is the smallest. Affect the order forentrainment: agents density> extraction pressure> time>extractiontemperature. Determine the puerarin consociation sibeline extraction withexcellent prescription for C1B2D3A2. In the influential factors in45℃temperature although is best, but in40℃temperature when theresults of itsvery close, for saving energy dissipation consideration, get the best extractiontechnology, extracting temperature is40℃,extracting pressure is35MPa,extracting time is4.5h, entrainment agents density is30%.Diabetic rats, blood sugar,24h urine protein increased significantly （P <0.01）, and diabetes group, puerarin extract treatment group than rats bloodsugar,24h urine protein obvious reducing （P <0.05or P <0.01）; Diabetic rats, renal hypertrophy index increased significantly, the puerarin extract kidneyhypertrophy treatment group extent lighter, statistically significant （P <0.05）;Light first show that the rats diabetes group glomerular change, capillary thethickened basement membrane, luminal stenosis, glomerular staining area HEexpanded, pueraria after treatment of the above abnormal extract are differentdegree of improvement （P <0.05or0.01）.ConclusionIn supercritical CO₂fluid extraction （SFE–CO₂） extraction of puerarinpuerarin, can finish at the same time distillation and extraction two process,extraction rate is high, the greatest degree keep effective ingredients, goodselectivity. By lowering blood sugar, pueraria lobata extract kidney hypertrophyindex, urine protein content of kidney pathological change and inhibition toimprove rats diabetes symptoms.