Study On The Significance Of The Expression Of LKB1and P53Proteins In Cervical Squamous Cell Carcinoma

ObjectiveDetecting the expression of P53and LKB1proteins in cervical squamouscell carcinoma and cervical intraepithelial neoplasia. Aimed to explore thepossible role in the pathogenesis and progression of cervical squamous cellcarcinoma.MethodsThe expression of P53and LKB1proteins were detected in60cases ofcervical squamous cell carcinoma and60cases of cervical intraepithelialneoplasia by immunohistochemistry method.20cases of cervical chronicinflammation tissue as control.ResultsImmunohistochemistry results showed that the positive rates of LKB1protein were25%（15/60cases） in the group of cervical squamous cellcarcinoma,and60%（6/10),53.3%（8/15）,42.8%（15/35）in the groups ofcervical intraepithelial neoplasia I, II and III respectively.100%（20/20）positive expression was observed in the group of chronic cervicitis. Thesignificant difference statistically was obteined among the groups of cervicalsquamous cell carcinoma, cervical intraepithelial neoplasia I、II and chroniccervicitis, but no significant differences statistically was observed between thegroups of cervical squamous cell carcinoma and cervical intraepithelialneoplasia III.（χ2=3.262，P=0.071）;Higher positive rates of LKB1protein was found in the groups of well-moderate differentiation and non-lymph nodemetastasis than that of in the groups of poorly differentiation （χ2=12.377，P=0.000）and lymph node metastasis（χ2=5.658，P=0.017）.Higher positive rate of P53protein was obtained (66.7%,40/60cases） inthe group of cervical squamous cell carcinoma than that of in the groups ofcervical intraepithelial neoplasia I and II， but no significant differencesstatistically was found between the groups of cervical squamous cellcarcinoma and cervical intraepithelial neoplasia III. no positive expression wasobtained in the group of chronic cervicitis（0/20）; Lower positive rate of P53protein was also observed in the group of cervical well-moderate differentiationsquamous cell carcinoma than that of in the group of poorly differentiation（χ2=5.284，P=0.022）. Significant lower positive rate of P53protein wasobserved in the group of non-lymph node metastasis than that in the group oflymph node metastasis（χ2=7.037，P=0.008）.ConclusionsAs a candidate tumor-suppressor genes, LKB1genes account for theinhibiting in the pathogenesis and the progression in cervical squamous cellcarcinoma; and there might be synergistic effect between p53genes lkb1genes in the progress of cervical squamous cell carcinoma.