Silencing Of SOCS1and IL-12Gene Cotransfered By Adenoviral Enhances DC-mediated Anti-laryngocarcinoma Immunity In Vitro

ObjectiveTo investigate the effects and the related immunological mechanisms ofdendritic cells(DCs) modified by SOCS1siRNA gene and interleukin-12gene onactivating and inducing cytotoxic T lymphcyte(CTL) as well as specificimmunitically killing tlaryngocarcinoma in vitro.MethodsDCs were derived from human peripheral blood mononuclear cells(hPBMC), modified by recombinant SOCS1siRNA adenoviral and IL-12adenoviral and then pulsed with tumor antigen of repeated freeze-thaw method.The IL-12and IFN-γlevels in culture supernatant of DCs and CTLs wereexamined by ELISA.ResultsDC were cultivated successfully and had special morphologicharacteristicistics. The rate of Ad-GFP carrying fluorescent expression is over90%. Flow cytometry results confirmed that the rate of phenotype of CD80andCD86expression rate of about40%, in line with the literature.The expression ofSOCS1protein in DCs were effectively decreased by being modified SOCS1siRNA and IL-12genetic while the expression of IL-12protein were increased.The secretion rate of IL-12factor was higher than SOCS1siRNA and IL-12transfection of single gene respectively in modified DCs which can prompt T cellproliferation activation significantly as well. IFN-γ was secreted constantly in DCand CTL, resulting in Hep-2. Conclusions（1）DC modified by SOCS1siRNA and IL-12gene which plused with larygnealcarcinomal antigene can increased the production of IL-12andI FN-γ.（2）DC modified by SOCS1siRNA and IL-12gene which plused with larygnealcarcinomal antigene can enhance the ability to stimulate proliferation of T cell,increase production of IFN-γ,IL-12by T cells and induce the stronger killing rateof CTL.