Protective Effects Of Xue Bi Jing Injection Against Ischemia Reperfusion Injury In Rats Hepatic

Objective:To investigate the protective effect of xuebijing injection against ischemia-reperfusion injury in rat liverand its mechanism.To expand its clinical purposes to provide the basis.Methods:108healthy SD rats were randomly divided into three groups:sham operation group (group A,n=36),control group (group B,n=36) and experimental group (group C,n=36).The rats in experimental group received xue bi jing injection (10mL/kg) in traperitoneally,qd,for3continuous days,(rats fed during regular water supply,12h lighting), the first30min to give the same amount of xue bi jing injection.The control group give physiological saline, dosage and method with the experimental group. The sham operation group were only flipped the intestinal canal and underwent a midline laparotomy for30min.Pringle’s method to establish the hepatic ischemia-reperfusion model, ischemia after30min remove blood vessels clip restore blood flow to the hepatic.2,6,12hour after reperfusion,venous blood samples and hepatic tissue samples were taken form easement.12rats were randomlychoiced in each group. Serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by the BeckmanDXC800auto maticbiochemistry analyzer. The Bax,Bcl-2and Caspase-3levels were measured by Immnohistochemistry.TUNEL method to detect hepatic cell apoptosis.Results:The values of serum ALT、AST、LDH in groupA were markedly down regulated than the other groups (P<0.05);ALT、AST、LDH in group C were significantly lower than in group B.The expression protein of Bax was higher in group B than in group C(P<0.05)and in group A (P<0.05).There were no significantly differences between group C and A (P>0.05).The expression protein of Bcl-2was lower in group A than in group C (P<0.01) and group B.There were no significantly differences between group C and B (P>0.05).The expression protein of Caspase-3was higher in groupB than in group C (P<0.05) and A (P<0.05).There were no significantly differences between group C and B (P>0.05).TUNEL method to detect hepatic cell apoptosis.The level of groupB was higher than group C (P<0.01)and A (P<0.01),group C was higher than group A (P<0.01).The histopathologic alterations under light microscope in group A were basically normal.In group B,with the time going on,the histopathologic changes gradually aggravated,the changes included different degrees of hepatic congestion,configuration destroy of hepatic sinusoid,degeneration and necrosis of liver cell.The changes mentioned in group C were milder than in group B.Conclusion:Xue bi jing injection can reduce Bax/Bcl-2proportion, reduce the Caspase-3protein expression and further restrain cell apoptosis,against ischemia reperfusion injury in rats hepatic.