The Expression Of CD28-B7in Perpheral Blood Mononuclear Cells Of Patients With Syphilis

Background and Objective: It’s normally thought that immune reaction mediated by cells plays an impotant role in the defence against infection in syphilis patients. The activation of T cell requires not only the antigenic stimulation sign, but also the subsidiary signal. Many researches show that CD28-B7are very important co-stimulatory molecules, which are necessary to specific cell immune reaction and are key to start and regulate the reaction. B7includes B7-1(CD80) and B7-2(CD86), they combine with CD28to transfer co-stimulatory signal and can affect the polarization of Th1/Th2. This study aims to investigate the significance of CD28-B7, co-stimulatory molecules of T lymphocytes in the onset, development and prognosis of patients with syphilis by detecting the expresstion levels of B7(CD80/CD86) in peripheral blood mononuclear cells and of CD28in CD4+T lymphocytes in syphilis patients and healthy volunteers as control.Methods:Flow cytometry and reverse transcription-polymerase (RT-PCR) technique were applied to detect the expresstion level of B7(CD80/CD86) in peripheral blood mononuclear cells and of CD28in CD4+T lymphocytes in syphilis patients and healthy volunteers as control.Results:The early LS patients had lower CD28(p<0.05), but the expression of CD80and CD86was not different from that of the control(p>0.05); the expression of CD28, CD80and CD86of exhibited significantly difference from that of the healthy people(p<0.05); compared with the early LS patients, the expression of CD28in the sero-resistant patients had no difference(p>0.05), but the CD80and CD86were significantly lower(p<0.05).Conclusion:The expression of CD80, CD86and CD28on peripheral blood mononuclear cells in syphilis patients was decreased, which impacted the CD28-B7co-stimulatory pathway and then the immune function of T cells were impaired, the imbalance of Thl/Th2appeared. This may be involved in the onset, development and prognosis of syphilis.