Model Improvement Of Abdominal Compartment Syndrome And Its Mechanism In Rabbit

ObjectiveTo establish rabbit model of abdominal compartment syndrome (ACS), Evaluate the impact of ACS on the cardiovascular, respiratory function and blood electrolyte level in rabbits and to observe the impact of long-lasting ACS to hemodynamic, hepatic function, renal function, antioxidation and blood electrolyte level; Evaluate the effects of growth-inhibiting hormone (somatostatin) on ACS.MethodsPart1:24New Zealand rabbits were separated into4groups equally:Normal group, ACS5group (IAP=5mmHg), ACS10group (IAP=10mmHg) and ACS20group (IAP=20mmHg). ACS model is produced by intra-abdominal bleeding (IAB) plus intra-abdominal hypertension (IAH). All data were recorded1hour after different IAP, including:cardiovascular parameters (LVSP, LVEDP,±dP/dt, SP, DP, HR, CVP), respiratory function (RR, PaO2, PaCO2,[HCO3-]), blood pH, and electrolyte level ([K+]). Part2:12New Zealand rabbits were separated into2groups equally:NS group (treat with normal saline), SOMATOSTATIN group (treat with somatostatin). IAH/ACS model is produced by intra-abdominal bleeding (IAB) plus intra-abdominal infusion with nitrogen gas until IAH reaches15mmHg. All data were recorded1-6hours later, including:hemodynamic (SP, HR, CVP), hepatic function (ALT), renal function (BUN), antioxidation level (SOD, MDA) and blood electrolyte level (pH,[Na+],[C1-],[Ca2+],[K+]).Results1. LVSP, LVEDP,±dP/dt, SP, DP, HR, RR, PaO2,[HCO3-], and blood pH of ACS20group were decreased significantly compared with normal group, CVP, PaCO2, and K+increased significantly (P<0.05). All data except RR and PaO2in ACS10group show same significant changes as that in ACS20group (P<0.05).But only LVSP and HR in ACS5group were reduced remarkably (P<0.05).2. Hemodynamic and antioxidation level decline as well as hyperkalemia and hypocalcemia (P<0.05) in long-lasting ACS. But there are no differences between NA group and somatostatin group.ConclusionIAB plus IAH may cause damage to cardiovascular, respiratory function, and lead to ACS in rabbit. And long-lasting ACS injures cardiovascular function of rabbit, and may be related to acidosis, electrolyte disturbances, and organizations oxidative damage caused by tissue ischemia and hypoxia. No apparent protective effects of somatostatin were observed on the occurrence and development of ACS.