Effect Of Silencing Podoplanin Expression On Biological Function Of DSCs In Human First-trimester Pregnancy

Objective To explore the expression level of transmembrane glycoproteinpodoplanin in decidual stromal cells(DSCs) at maternal-fetal interface in thefirst-trimester pregnancy and its effect on the biological function of themselves.Methods All the deciduas and villis were collected from the early pregnancy.DSCs and trophoblasts were isolated by digesting and density gradient centrifugationand then cultured. After that, the expression levels of podoplanin and its receptorCLEC-2were detected applying the methods of immunohistochemistry,immunocytochemistry and flow cytometric analysis. In order to further elucidate theeffect of podoplain on biological functio of decidual stromal cells, the expression ofpodoplanin in DSCs was interfered with siRNA technique, and then the cells viability,proliferation and apoptosis were respectively detected by MTT assay, BrdU assay andannexin V-FITC apoptosis detection kit.Results The DSCs and trophoblast cells of the primary culture are all positivelystained with anti-podoplanin or anti-CLEC-2antibodies. Besides, the expression ofpodoplanin in DSCs appear a much higher level than that of the trophoblast cells, butCLEC-2expression are low in both DSCs and trophoblasts. Podoplanin silencingproduced effect of inhibition to the viability, proliferation and invasiness of DSCs, butpromoted the apoptosis.Conclusion The data above-offered suggests that podoplanin expression caninfluence the biological function of human DSCs and maybe play a intermediate rolein the signals transduction of regulating the DSCs’ proliferation, apoptosis andinvasiness, and thus will be beneficial to the maintenance of pregnancy.