Reasarch Of Dl-3n-butylphthalide Influences On Antioxidant Capacity In Aging Mice Brain

Objective To detect the effects of dl－3n－butylphthalide (NBP) on antioxidantcapacity in aging mice brain and probable mechanism of action.Methods28aged male C57BL6mice were divided into two subgroups randomly:control animals (n=14) and treated animals(n=14),10young male mice were taken asyoung controls(n=10).A group of aged animals received NBP dissolved in soybean saladoil by intragastric administration, the other group and the young group received soybeansalad oil without NBP, The administration period lasted for12weeks. Arriving at thetime,Blood samples were collected from the retro-orbital plexus and were centrifuged toobtain serum. Then, all mice were killed by cervical transaction, and the brain tissue wasremoved immediately. Serum and tissue were stored at–80℃nitrogen until analyses. Todetect protein carbonyl, malonaldehyde (MDA) and total antioxidant capacity of all braintissue,and to measure brain tissue total GSH-Px activity, serum GSH-Px activity, andmitochondrial GSH-Px activity.Results Compared with the young group, level of protein carbonyl, MDA allincreased（P＜0.05）, and total antioxidant capacity, brain total GSH-Px activity, serumGSH-Px activity, and brain mitochondrial GSH-Px activity all descreased of the old mice（P＜0.05）. Compared with the old control group, level of protein carbonyl, MDA alldescreased （P＜0.05）, brain total antioxidant capacity, serum GSH-Px activity, and brain mitochondrial GSH-Px activity all improved（P＜0.05）, brain total GSH-Px activity werenot changed of the treated group（P﹥0.05）.Conclusion NBP can reduce oxidative markers, such as protein carbonyl, MDA, byimproved total antioxidant capacity, serum GSH-Px activity, and brain mitochondrialGSH-Px activity.