Comparation Of The Two Kinds Of Magnetic Nanoparticles Coating With Different Polysaccharide

This subject will use Angelica polysaccharide as a carrier material to prepareAngelica polysaccharide magnetic nanoparticles, and in the same time compare thetwo mature reference preparations in the characters in vivo and vitro. In this paper, wewill evaluate the feasibility and the special advantages of Angelica polysaccharide asa carrier of magnetic nanoparticles. This paper mainly includes five aspects: comparedwith the preparation method, quality analysis, characterization, body distribution andloading nature.Part One The three kinds of magnetic nanoparticles coating withdifferent polysaccharideTaking Angelica polysaccharide as raw material, the total polysaccharide wasextracted by boiling water and pured after removing tannin by Calcium hydroxide.Phenol-sulphuric acid method was used to determine the content of polysaccharide.The three kinds of magnetic nanoparticles were prepared by chemical coprecipitation.By comparison with appearance, water-solubility, magnetism response and particlesize, the purpose is aim to generally evaluate the property of the product. Magneticdecantation method and Dynamic dialysis method were applied to purify the twokinds of magnetic nanoparticles, and obtained two kinds of refining magneticnanoparticles namely Asp-Fe₃O₄and Dex-Fe₃O₄, respectively. Phenol-sulphuric acidmethod was used to determine the content of polysaccharide and byo-phenanthrolinemethod was used to determine the content of iron content. The experimental resultsshowed that the polysaccharide content of the extractum is about67.72%, theAngelica polysaccharide magnetic nanoparticles and the dextran magneticnanoparticles also have a good character in vitro, such as good water-solubility, theuniform particle size and strong magnetism, but the chitosan magnetic nanoparticleshas a low water-solubility and agglomeration in vitro. the Angelica polysaccharidemagnetic nanoparticles of the content of Fe₃O₄is about68.90%and thepolysaccharide content is about16.13%, the dextran magnetic nanoparticles of the content of Fe₃O₄is about49.50%and the polysaccharide content is about39.80%. Bypreliminary determination, the Angelica polysaccharide can be used as materialcarrier of the magnetic nanoparticles, and successfully prepared the Angelicapolysaccharide magnetic nanoparticles, the dextran magnetic nanoparticles will besuitable as a reference preparation and the chitosan magnetic nanoparticles will not besuitable as a reference preparation.Part Two Comparation of the two kinds of magnetic nanoparticlescoating with different polysaccharide in vitroMagnetic nanoparticles were prepared by chemical co-precipitation which wasdetermined by the first part, and their physical and chemical properties were observedto study the stability of the Angelica polysaccharide as material carrier of themagnetic nanoparticles. The connections was determined by the infraredspectroscopy（FTIR）, the structure and purity were detected by using the X-raydiffraction（XRD）, the sizes and shapes were detected by using the transmissionelectron microscopy（TEM） and the laser particle size analyzer（DLS）, the magneticproperties was determined by the vibrating sample magnetometer（VSM）. The resultsshowed that FTIR proved Fe₃O₄were successfully coated by the two kinds ofpolysaccharide, and forming a stable chemical bond. XRD showed the maincomponent was Fe₃O₄crystals of high purity. TEM and DLS showed that both the twokinds of magnetic nanoparticles possessed small particle size and good dispersion, themean partical size was79.2nm and87.9nm, respectively. Therefore the two kinds ofmagnetic nanoparticles owned good superparamagnetism. The saturationmagnetization was21emu/g and22emu/g, respectively. By comparing the two kindsof magnetic nanoparticles in vitro, a preliminary judge for the study that the Angelicapolysaccharide magnetic nanoparticles could showed good productive in vitro, andwas suitable for the reseach in vivo experiment. Part Three Comparation of the two kinds of magnetic nanoparticlescoating with different polysaccharide in targetdistribution in vivoTo evaluate the stability and distribution of the two kinds of magneticnanoparticles by the metabolic situation. Healthy male SD rats were used as the modelanimals with the tail vein administration. The two groups of rats were settled in themagnetic field and not，and we observed the iron disposition in rats. Using theatomic absorption spectrometry to test the content of the iron in different organs, andto analyze the iron content of the two kinds of magnetic nanoparticles in heart, liver,spleen, lung and kidney. Using the Prussian blue staining method to test thedistribution of the content of iron, and to analyze the iron content of the two kinds ofmagnetic nanoparticles in liver and kidney. The experimental results showed that thetwo kinds of magnetic nanoparticles have passive targeting in vivo without magneticfield, the dextran magnetic nanoparticles could accumulate in liver, spleen and lung,the Angelica polysaccharide magnetic nanoparticles could only accumulate in spleenand lung. The two kinds of magnetic nanoparticles have active targeting in vivowithin the magnetic field, they could accumulate in liver. By comparing the two kindsof magnetic nanoparticles in vivo, a preliminary judge for the study that the Angelicapolysaccharide magnetic nanoparticles showed good stability in vivo, and be suitablefor the study of drug carrier.Part Four The performance ofthe two kinds of magnetic nanoparticles as drug carriesUsing the reverse microemulsion method,5-FU was used as the model drug andglutaraldehyde as the crosslinker to prepare the two kinds of magnetic nanoparticlesas drug carriers namely5-FU-Asp-Fe₃O₄and5-FU-Dex-Fe₃O₄.To investigate theparticles size distribution, load rate, the encapment efficiency, the releasing featureand the magnetism response of the two kinds of magnetic nanoparticles after being carried drug. The sizes and shapes of the carried drug were detected by using the laserparticle size analyzer. The load rate and the encapment efficiency of the carried drugwere detected by using the UV spectrophotometry. The releasing feature of the carrieddrug was detected by using the apparatus suitability test.The magnetism response ofthe carried drug was detected by using the dipping method. The experimental resultsshowed that the sizes and shapes increased after loading the drug, and about260nm,but the dispersion become trail off. The load rate of the5-FU-Asp-Fe₃O₄was8.98%,and the entrapment efficiency was39.30%. The load rate of the5-FU-Dex-Fe₃O₄was14.31%, and the entrapment efficiency was62.77%. The drug release process of the5-FU-Asp-Fe₃O₄was slow-release, and the drug release process of the5-FU-Dex-Fe₃O₄was controlled-release. The two kinds of magnetic nanoparticlesshowed good magnetism response. To sum up, the Angelica polysaccharide could useas a carrier material to prepare the Angelica polysaccharide magnetic nanoparticles,and used in targeted drug delivery system.