Effect Of Latanoprost Free Acid On Proliferation And Expression Of CTGF In Human Tenon Capsule Fibroblasts

Objective: Long term use of anti-glaucoma medication, the filtering surgery ofglaucoma patients may be available for scaring. Prostaglandin drugs are currentlyconsidered as the most potential and effective topical drugs used in clinic for treatingprimary open angle glaucoma extensively. This study intends to investigate theproliferation and expression of connective tissue growth factor (CTGF) of latanoprostfree acid (Lat-FA) works on human Tenon capsule fibroblast cells (HTFs), investigatethe related regulation mechanism further.Methords: Act in accordance with the Helsinki Declaration, choose the cataractpatients who are without the history of using anti-glaucoma medications, acquired theTenon tissues during surgery, culture primary HTFs. Optical microscope and Liustaining are used to observe cells.1. HTFs dealed with latanoprost free acid (Lat-FA) of the different times anddifferent concentration, CCK-8kit detect the proliferation of cells.2. HTFs dealde with Lat-FA, ELISA detect the difference of expression ofCTGF. 3. HTFs dealed with Lat-FA and inhibitors of MAPK signaling pathway, ELISAdetect the difference of expression of CTGF.Results:1. Different concentrations Lat-FA works on HTFs,0ng/ml、0.5ng/ml、5ng/ml、50ng/ml、500ng/ml、5000ng/ml, measure the OD values are:0.334±0.011,0.336±0.010,0.338±0.009,0.359±0.011,0.389±0.018,0.424±0.021, comparedwith the control group, P values of0.5ng/ml and5ng/ml groups are:0.665,0.375,there is no significant difference between these three groups in statistics; the othergroups P values are all <0.05, and the difference is significant in statistics. Lat-FAworks on HTFs in different times(24h,48h,72h), measured the OD values were:0.349±0.030,0.361±0.032,0.379±0.039, compared with24h group,48h group P valueswas0.088, there is no significant difference between the two groups in statistics;compared with the other two groups, there is statistically significant difference in72hgroup.2. ELISA measure the expression of CTGF after HTFs were dealed with Lat-FAafter24hours. Control group:83.577±2.752ng/L, Lat-FA group:108.710±3.654ng/L. the Lat-FA group compare with the control group with statistically significantdifference (P<0.05).3. Before Lat-FA dealed, HTFs were dealed with three different inhibitors ofMAPK signaling pathway0.5hours. There are four groups include: Lat-FA group,Lat-FA+SP600125group, Lat-FA+SB203580group and Lat-FA+UO126group. ELISAmeasure the expression of CTGF are as follows:108.710±3.654ng/L,75.199±3.040ng/L,84.507±4.239ng/L,99.091±6.359ng/L; the three used differentMAPK signaling pathway specific inhibitors, compared with the Lat-FA group, thereare significant differences in statistically (P<0.05).Conclusion: Lat-FA has a certain effect of proliferation in HTFs, the effect may relate to time and concentration; Lat-FA could up-regulate the secretion and expression ofCTGF in HTFs; and Lat-FA up-regulate the secretion and expression of CTGF inHTFs may mediated through MAPK signaling pathway.