Anti-tumor Function Research On Containing Serum Of Dioscorea Bulbifera L. And Dioscorea Bulbifera L.Compatibility With Glycyrrhiza Uralensis Fisch

Objective:1.By studying the effect which containing serum of Dioscorea bulbifera L. and Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch. took on proliferation and apoptosis of different tumor cells in vitro, to observe the anti-tumor effect of them.2.By comparing the anti-tumor effect of Dioscorea bulbifera L. and Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch., to observe the effect of Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch.Methods:This subject used vinous modern experimental techniques,such as serum containing experimental methods in serum pharmacology of Traditional Chinese medicine,cells cultured in vitro,cell morphology observation,cell proliferation detected by MTT method and cell apoptosis detected by Annexin V-FITC/PI French flow cytometry,etc.The data obtained in the test were analysised by SPSS17.0software.Results:1.Containing serum screening test results showed that containing serum collected from rats gavaged for five days performed strong inhibition of human hepatocellular carcinoma cells in vitro;containing serum collected from rats gavaged for three days performed strong inhibition of rat breast cells in vitro.2.The tumor cell morphology experiment showed that form changed in both tumor cells after serum-intervention,and the degree of change showed significantly positive correlation with the proportion of intervention serum and intervention time.The greater the proportion of serum, the more obvious changes;the longer the intervention time, the more obvious changes.3.The results of MTT assay Cell proliferation experiment showed that the containing serum of Dioscorea bulbifera L. and Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch. inhibited both tumor cells proliferation in certain extent.①The rate of inhibition on human hepatocellular carcinoma cells in vitro were containing serum of Dioscorea bulbifera L.18.53%,containing serum of compatibility12.95%;②The rate of inhibition on rat breast cancer cells in vitro were containing serum of Dioscorea bulbifera L.37.80%,containing serum of compatibility31.34%.4.The result of flow cytometry apoptosis detectionshowed that the containing serum of Dioscorea bulbifera L. and Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch. had a certain impact on both tumor cells apoptosis.①The apoptosis rate of human hepatocellular carcinoma cells in vitro were containing serum of Dioscorea bulbifera L.24.03±3.36%, containing serum of compatibility21.86±2.93%;②The apoptosis rate of rat breast cancer cells in vitro were containing serum of Dioscorea bulbifera L.43.47±2.07%,containing serum of compatibility34.73±2.91%.Conclusion:1.The containing serum of Dioscorea bulbifera L. and Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch.,not only showed direct inhibition to the growth of both tumor cells,but also induced apoptosis in both tumor cells effectively.And they had significant anti-tumor activity in vitro.2.The containing serum of Dioscorea bulbifera L. Showed better effect on inhibiting both tumor cells proliferation and inducing both tumor cells apoptosis, it indicated that the efficacy declined after the compatibility of Dioscorea bulbifera L.and Glycyrrhiza uralensis Fisch.