| Antarctic Krill is a kind of zooplankton that lives in the Antarctic ocean. It enjoys thelargest quantity and is one of the most successful species. It is believed that the amount ofAntarctic Krill is between0.65billion tons and1billion tons. Related research has proved thatAntarctic Krill is good to people’health. The functions of its oil are comprehensive, stable andlong-term, without side effects,and the underlying fish resource and economic advantage isbeyond your imagine! Therefore, applications of the Antarctic krill oil on medicine, food,agricultur and industry are widespread. However, the research on Antarctic krill oil is still onlyunder way. The deep development of Antarctic krill oil processing technology, particularly thedevelopment of high-purity Antarctic krill oil preparation method has important social andeconomic value in China.This papers is to find a way to distill Krill oil and analyse its majorbases.It studied the extraction of Antarctic krill oil with alcohol.Including immersion,distillation, drying, and finally obtained the Antarctic krill oil of high purity. This research wasdivided into three parts.Part1:The extraction of oil in Antarctic krill by organic solvent was investigated in this paper.Extraction process was studied through single factor’ s test and orthogonal experiment, and theoptimization conditions of it were listed as follows:The absolute alcohol was suitable asextraction solvent. And the yield was19.60g/100g (dry basis),when it was extracted at65℃for3h, powder of krill: complex of organic solvent ratio1:9(w:v).Part2: Fatty acid constituents in krill oil were analyzed and determined by gaschromatography.The main result showed that:The major fatty acid constituents wereC16:0(21.3%),C14:0(10.11%)ofSFA,C18:1(13.3%)andC16:1(5.54%)ofMUFA,DHA(11.6%)and EPA(15.0%) of PUFA.Part3:Study of the Effect of Euphausia Superba Oil on Model of Ethanol-induced Fatty Liverin Mice Objective Investigate the effect of Euphausia Superba oil on model of ethanol-induced fattyliver in mice. Methods A total of70mice were fed with fat-rich forage plus ethanol infusionfor30days in order to make model success. The injured mice were randomly and averagelydivided into A、B、C、D groups. The mice in group A served as natural recovery group. Themice in group B、C and D were treated with high, medium, low dose of Euphausia Superba oilfor10days and detected related biochemical and pathological index, respectively.Results Compared with group A, the levels of serum lipids including the triglyceride(TG),total cholesterol (TC) decreased and the levels of glutamic-oxalacetic transaminease(AST), glutamic-pyruvic transaminase (ALT) also decreased in Euphausia Superba oil groups(P<0.01or P<0.05).After treatment of Euphausia Superba oil, the levels of TG and TC inhepatic tissue were lower than those in natural recovery group (P<0.01or P<0.05). The frozensections of fat dyeing results show that the accumulation of lipid in liver cell dropedsignificantly in Euphausia Superba oil groups. Conclusion In this study condition, EuphausiaSuperba oil has therapeutical effect on ethanol-induced fatty liver in mice. |
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