The Preliminary Study Of The Roles Of Lymphocyte Subpopulation In Parkinson’s Disease

Objective:To detect the percentage of Parkinson’s disease (PD) patients’peripheral blood lymphocyte subpopulations and analyze the correlation between the change of the percentage and the course of the disease in order to provide a scientific basis for the immunotherapy. To analyze the distribution of PD patients’lymphocyte subpopulations after treatment with alpha-Synuclein (a-Syn) and explore the possible mechanisms.Method:1. A case-controlstudy was conducted. Thirty-four PD patients and twenty healthy controls (HC) were enrolled. Peripheral blood samples were collected with the written informed consent. PBMCs were isolated by Ficoll. Then the percentage of CD3+, CD3+CD4+, CD3+CD8+and CD19+in lymphocytes was detected by FCM. The correlation between the percentage of those subpopulations and the course of the disease was analyzed.2. Ten age-and gender-matched subjects from PD and HC groups were selected respectively. PBMCs from above individuals were isolated and then were treated with a-Syn. Seventy-two hours later, the distribution of lymphocyte subpopulations were detected by FCM.3. FCM analysis was performed to evaluate the effect of a-Syn on lymphocyte apoptosis.4. JC-1staining and FCM were used to detect the influence of a-Syn on lymphocyte mitochondrial potential (ΔψM).Results:1. The percentage of CD3+T in PD patients was higher than in HC group without statistical significance (P>0.05). The percentage of CD3+CD8+T in PD group was much higher than HC group, the difference was statistically significant (P<0.05). Correlation analysis between the percentage of CD3+T, CD3+CD8+T and the H-Y scores showed that the former was positive correlation with the latter (r=0.530, r=0.597, P<0.05).2. PBMCs were treated by a-Syn at final concentration of10μg/ml for72hs, the percentage of CD3+CD4+T in PD group was lower than that in HC group with statistical significance (P<0.05).3. After treatment on PBMCs with a-Syn at10μg/ml for48hrs, FCM results revealed higher percentage of lymphocyte apoptosis in PD group (28.50±1.20%) than that in HC group (7.00±0.05%) with significant difference (P<0.05).4. After treatment on PBMCs with a-Syn at10μg/ml for48hrs, the detection of ΔψM by JC-1staining showed higher level of decreased ΔψM in PD group than that in HC group with significant difference (P<0.05).Conclusion:1. The percentage of CD3+CD8+T increased in PD patients, which may be a dangerous factor to PD progress. And it was association with motor disturbance in PD patients.2. a-Syn may induce lymphocyte apoptosis in PD patients via decreasing the AyM.