| Objective:1. To compare the concentrations of interleukin-1β(IL-1(3), interleukin-6(IL-6), interleukin-10(IL-10) in vitreous samples from patients with proliferative diabetic retinopathy (PDR) and normal group patients who were accidently died without the reason of medicine and disease.and to analyze their interrelationship and influencing factor.2. To determine the role of IL-1β,IL-6and IL-10in the pathogenesis of PDR and to provide theoretical basis for preventing and treating pathients with early stage of PDR.Methods:Twenty five cases (26eyes) with PDR were recruited into this study. All cases were diagnosised in the eye department of Tianjin medical university general hospital from March2011to January2012. The cases with PDR included12(12eyes) males and14(15eyes) females. The age of cases ranged from38to73years, and the mean age was55±13.2years. The histories of diabetes mellitus (DM) were from2to30years in cases and the mean was13.8±2.9years. The symptoms of blurred vision and dark shadow had2to24months and the mean was5.6±2.5months in cases. All cases were graded by the classification of diabetic retinopathy (DR) which issued by the committee of ocular fundus diseases of China in1984. The cases included6(6eyes) patients with stage ⅣV(23.08%),9(10eyes) with Stage V (38.46%),10(10eyes) with Stage VI (38.46%). Twenty six vitreous samples were obtained via pars plana vitrectomy (PPV) operations. Eight controls (8eyes) who were accidently died and without any history of disease were selected into this study. There were4(4eyes) of males and4(4eyes) of females. The age of controls ranged from24to40years and the mean age was36+6.8years. Vitreous samples were obtained by a25gauge disposable syringes via pars plana. All samples were stored in eppendorf tube at-20℃immediately after obtained. The concentrations of IL-1β,IL-6and IL-10in vitreous samples were measured by enzyme-linked immunosobent assay (ELISA). All data were analyzed with SPSS17.0and concentrations of IL-1β, IL-6and IL-10 were represented by (mean±standard deviation). The data were coincidenced with gauss distribution tested by Shapiro-Wilk test and the data were regular tested with homogeneity test for variance. The t-test.was used to analyze the difference of the concentrations of IL-1β, IL-6and IL-10between two groups. One-factor analysis of variance is applied to compare the difference of the concentrations of IL-1β, IL-6and IL-10among stages. The difference of the concentrations of IL-1β, IL-6and IL-10interclass was compared with t-test. Linear regression analysis was applied to analyze the relevance among the concentrations of IL-1β, IL-6and IL-10in PDR. The difference was considered with significance when P<0.05.Results:1. The means of IL-1β,IL-6and IL-10concentrations in vitreous from cases were14.916±3.125pg/ml,6.867±1.352pg/ml,224.789±43.801pg/ml respectively. The mean of IL-1β,IL-6and IL-10concentrations in vitreous from controls were11.991±1.843pg/ml,5.275±0.838pg/ml.159.375±43.692pg/ml respectively. The difference of IL-1β,IL-6. IL-10concentrations of vitreous was statistically significante between cases and controls (t=3.516,2.788,3.696; P=0.009,0.002,0.000).2. There was a significantly positive correlation between the IL-1β and the IL-6in cases group (r=0.433. P=0.013) and the regression equation was Y=1.110X+7.291pg/ml. There were significantly negative correlation between the IL-10and the IL-1β and between the IL-10and the IL-6in cases (r=-0.872,-0.635; P=0.000,0.000). The regression equations were Y=-11.479X+395.146pg/ml and Y=-20.382X+363.884pg/ml. There were no significantly correlation among the IL-10,the IL-1β and the IL-6in controls (r=-0.045,-0.005,0.275; P=0.916,0.991,0.509).3. The means of IL-1β concentrations in vitreous from patients with PDR stage Ⅳ, Ⅴ and Ⅵ were11.004±1.196pg/ml,14.066±1.100pg/ml,18.114±1.663pg/ml respectively. The means of IL-6concentrations in vitreous from patients with PDR stage Ⅳ,Ⅴ and Ⅵ were.6.552±0.876pg/ml,6.230±0.676pg/ml,7.693±1.708pg/ml respectively. The means of IL-10concentrations in vitreous from patients with PDR stage Ⅳ,Ⅴ and Ⅵ were265.058±37.325pg/ml,237.085±32.265pg/ml,186.080±22.323pg/ml respectively. The difference of IL-1β concentrations had statistical significance among the PDR stage Ⅳ.Ⅴ and VI (F=53.897. P=0.000). There were statistically significant differences between the stage VI and Ⅳ.Ⅵ and Ⅴ,Ⅴ and Ⅳ(/=6.420,9.101,5.223; P=0.000.0.000.0.000). The difference of IL-6concentrations of vitreous were statistically significant among the PDR stage Ⅳ,Ⅴ and Ⅵ (F=3.857, P=0.036). There were statistically significant difference between the stage VI and IV(t=2.519; P=0.011) and no statistically significant differences between the stage VI and V and between Ⅴ and Ⅳ (t=1.507.-0.827, P=0.077,0.211). The difference of IL-10concentrations of vitreous had statistical significance among the PDR stage Ⅳ. V and VI (F=14.475, P=0.000). There were statistically significant differences between the stage Ⅵ and Ⅳ, Ⅵ and Ⅴ (=-4.111,-5.347; P=0.000,0.000) and there were not statistically significant difference between the stage Ⅴ and Ⅳ (t=1.586;P=0.068).Conclusion:1. The IL-1β. IL-6and IL-10may play important roles in PDR pathogenesis. The Inflammation and Immunologic mechanism could be enhanced by IL-1β, IL-6and could be lessen by IL-10. The IL-1β,IL-6and IL-10concentrations in vitreous are significantly elevated in PDR patients. Inflammation and Immunologic mechanism may play an important role in pathologic processes and progression of PDR.2. Positive correlation between the IL-1β and the IL-6concentrations of vitreous with PDR indicates that they have the same effects in the Inflammation and Immunologic mechanism of PDR. Negative correlation between the IL-10and the IL-1β and between IL-10and IL-6in PDR indicates that IL-10could restrain the effects of IL-1β and IL-6.3. The higher stage of PDR may have the higher concentration of IL-1β and IL-6,and have the lower concentration of IL-10in vitreous. Comparatively absence of IL-10in vitreous might play an important role in development of PDR. The stage of PDR could be estimated by IL-1β, IL-6. and IL-10concentrations in vitreous. |
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