The Exptession Suuvey Of FGF Signalling Pathway In The Human Embryonic Tooth Germ

Tooth development is a very complex and delicate process, which is a result of epithelial tissue and mesenchymal tissue. Signal network system made by a large numbers of signaling molecules is involved in controlling this process. Using mice as the regular model organism for research, the mechanism of their tooth development has been studied thoroughly, but the research on the signal-regulated mechanism of human tooth development is still at early stages.In order to investigate the possible molecular mechanism of FGF signaling pathway during human tooth germ development, this experiment was first to select several genes of FGF signaling pathway and detected their expression pattern in the early stage of tooth germ development. Those genes included FGF3, FGF4, FGF7, FGF8, FGF9and FGF10. Through the comparison of expression pattern of these genes in the early stage of mice tooth germ development, so as to speculate and analyze the possible potential FGF signaling pathway mechanism in human tooth germ.Base on the research, we find the expression pattern of these genes has many similarities and differences among mice and human during the bell stage, bud stage and cap stage of tooth germ. We have some examples as following, FGF4is not limited express in the enamel knot, but in all the inner enamel epithelium. FGF7cannot be seen its expression in mice tooth germ, but it shows strongly expression for each stage of human tooth germ development. FGF10has obvious expression in mice test, but only show a little weak expression in human tooth germ.According to the result of this experiment, we believe that the control mechanism of FGF signaling pathway in the course of human tooth germ development has some similarities of the mice. But some genes such as FGF10, which function may be replaced by other signaling molecules in the development course. And FGF7plays an important part in the course of human tooth germ development. What we can be sure, FGFs signaling pathway plays an important part in the course of human tooth germ development.