| The2.7kg dried leaves of Morus alba Linn. was extracted by ethanol, concentratedalcohol extract, water dispersion, extracted with petroleum ether, ethyl acetate and n-butanolrespectively petroleum ether extract obtained107.5g, ethyl acetate extract12.4g, n-butanolextract of11.9g. The2.7kg dried leaves of Morus alba Linn. was extracted by water,concentrated and extracted with n-butanol, n-butanol extract of13.8g. Use of columnchromatography, HPLC separation and recrystallization of petroleum ether extract and ethylacetate extracts were isolated, purified, obtained22single compounds. By spectral propertiesand the literatures of their structures were identified: n-tetratriacontanol (1),-stigmasterol(2), solanesol (3), sucrose (4),(6S,9R)-roseoside (5),5α,6α-expoxy-β-ionone-3-O-β-D-gluco-pyranoside (6),5,5′-dimethoxylaricir-esinol-4′-O-β-D-glucopyranoside (7), adenosine (8),bridelionoside C (9), kaempferol-3-O-β-sophoroside (10), kaempferol-3-O-α-L-rhamnopyra-noside-(1→2)-β-D-glucopyrano-side (11), Scopolin (12), icariside B (13), luteolin-7-O-β-D-glucoside (14), kaemp fero-3-O-α-L-rhamnoside (15), scopoletin (16), kaempferol-3-O-β-D-glucoside (17), esculetin-7-O-β-D-glucoside (18),3β-hydroxy-5α,6α-epoxy-β-ionone-2α-O-β-D-glucopyranoside (19),(-)-syringaresinol-4-O-β-D-glucopyrano-side (20), ethyl-β-D-glu-copyranoside (21), gallic acid (22). The compounds6,7,10,11,19,21were isolated fromthis plant for the first time.On the basis of dried leaves of Morus alba Linn. petroleum ether extract and ethylacetate extract separation result, The main constituents of petroleum ether extract are fattyclass and sterides, whice n-tetratriacontanol (1) and-stigmasterol (2) of content is Morusalba Linn. leaves petroleum extract weight of3.4percent and2.3percent; the most abundantof the ethylacetate extract are flavonoid glycosides and Ionone glycoside, whice flavonoidglycosides and Ionone glycoside of content is Morus alba Linn. leaves petroleum extractweight of1.0percent and1.1percent. |
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