The Research On The Expression Of Caspase-9and MMP-3and NGH After Traumatic Brain Injury In Rats

Obiective: To study the expression changes of Caspase-9, MMP-3and NGB in ratswith traumatic brain injury and the relationship with brain injury and its forensicsignificance. Methods: Brain injury model was established by the freefall epiaural hitmethod,and different time periods expression of Caspase-9and MMP-3and NGBaverage optical density, positive area ratio and serum of Caspase-9content was detectedby immunohistochemical SP method, ELISA, and image analysis observation. The datahas been treated statistically. Results: ELISA results showed that the expression ofCaspase-9levels in serum after traumatic brain injury in rats showed rise-the peakperiod-the gradual decline of the change; compared with the normal control group, thecase group with brain injury after12h,1d,2d,3d, and5d group showed statisticallysignificant (P<0.05): the value was significantly enhanced at12h after injury (adjacent tothe previous group, P<0.05),which up to30.56±2.77ng/ml, after injury1d serum ofCaspase-9levels reached a peak of45.23±3.31ng/mL, then decreased gradually afterinjury7d (13.09±2.39ng/mL) it reduced to the normal range. The immunohistochemicalstaining image analysis results showed that rats after traumatic brain injury, Caspase-9,NGB and MMP-3ratio in the mean optical density value and positive area of expressionchanges showed rise-the peak period-the gradual decline regularity changes. Expressionof Caspase-9,MMP-3and NGB are separately sensitive in1d,3d and12h after headinjury. Conclution:1) Expression of Caspase-9, MMP-3and NGB showed a rise-thepeak period-the gradual decline regularity changes. Combined with three indicators havereference value to detect the early brain damage and the longer period.2) ELISAdetection provides a new experimental tool for the accurate inference of injury time andbrain damage identification.