Preparation Of Origanum Oil Colon-targeting Tablets And Study On The Stability

Oregano (Orgainum vulgare L.) of Labiatae Origanum perennials, has heat solution form and water swelling effect. Modern pharmacological research shows that oregano has anti-bacterial, anti-inflammatory, analgesic, regulating immunity effects on the cardiovascular system, respiratory system and other aspects, attending in cold, headache and body pain acute and chronic enteritis and abdominal pain. Liao Fang of oregano essential oil on Shigella bacteriostatic and bactericidal effects, results of oregano essential oil on infection Lapel within bacillary dysentery, Shigella flexneri F2a mouse has a protective effect on different bacteria, Bacillus dysenteriae has obvious bacteriostatic and bactericidal action. Deng Xuehua from origanum volatile oil of identification of69components, where in the thymol, carvacrol, on o-cymene, caryophyllene, terpineol and other six major components of74.29%of total content, its main effective antibacterial constituents of thymol, carvacrol accounted for13.38%and37.80%for the elucidation of oregano antibacterial material foundation provided some scientific basis.1、 oregano oil HP-β-CD preparation with orthogonal test method, the inclusion complex yield of volatile oil inclusion rate as evaluation index, inspected the inclusion temperature, oil and HP-β-CD proportion, the inclusion of saturation effect, finally determine inclusion temperature40℃, oil and HP-β-CD ratio of1:8. inclusion time40minutes is the best inclusion process.2、 HPLC method for the determination of oregano oil-HP-β-CD thymol, carvacrol, on Cymene methodology to investigate the Thermo BDS HYPERSIL C18column (4.6mm250mm,5m); acetonitrile-water (43:57) as mobile phase; the column temperature:35℃; the detection wavelength272nm; velocity::1.0ml min-1; sample size:50μ1. Results:in the chromatographic conditions, carvacrol on0.2-4.0μ g range showed good linear relationship, the regression equation was Y=525.7652X-12.2981r=0.9994; thymol in0.1-2.0μg range showed good linear relationship, the regression equation was Y=752.6134X-2.8428r=0.9999; p-cymene in0.1-2.0μg range showed good linear relationship, the regression equation was Y=1265.1478X-35.4011r=0.9999; conclusion:the HPLC method for the determination of oregano oil HP-β-CD thymol and carvacrol, p-cymene content, this method is simple, rapid, accurate3. Origanum oil-colon-targeting tablets preparation:mix0.2%talcum powder,0.2%microcrystalline cellulose and oregano oil HP-β-CD powder, then directly tabletting of appropriate hardness of the tablet core, with each index component of in vitro release rate and absorption rate of tablet coating formulation optimization, finally determined to EC: EudragitS100(7:3),10%DEP, coating weight gain4%as the coating formulation. The results show that, under the conditions of preparation of3batches of samples can achieve colon-specific release purposes, and has stable release properties.4、 Origanum oil of colon targeting stability study Bovine to volatile colon targeting coating for stability study. The oregano essential oil colon targeting coating sheet through air, light, temperature (40℃,60℃), and high humidity (25℃and25℃, relative humidity75%, relative humidity92.5%) and other factors influencing the test, as well as40℃, relative humidity75%conditions for3months to study the stability. The results show that the agents should be in room temperature sealing, stored away from light, effectively the provisional period of2years.