Effects Of Methyl Methacrylate On Cardiomyocyte Apoptosis And The Expression Of Bcl-2and Bax

ObjectiveTo investigate the effects of methyl methacrylate on cardiomyocyte apoptosis ofnewborn Sprague-Dawley rats by detecting the cell viability and apoptosis intex aftertreated by methyl methacrylate with different concentrations，and by checking theexpression of Bcl-2and Bax.MethodsCardiomyocyte was prepared from newborn rats and divided into sixgroups.Group1was defined as the control group，group2to6were treated bymethyl methacrylate with0.5、1.0、2.0、4.0、8.0mg/ml for2h respectively.CCK-8assay was used to detect cardiomyocyte viability and TUNEL cell staining forapoptosis,the expression of Bcl-2and Bax was detected by western blotting.ResultsMethyl methacrylate incubation for2h decreased the cell viability and triggeredapoptosis in a concentration dependent manner.Compared with the control group, thecell viability was significantly decreased to（71.0±6.6）%in group4、（55.3±6.0）%in group5、or（34.0±7.0）%in group6（P＜0.01），theapoptosis index was evidently increased to （15.33±0.43）%in group4、（32.88±0.30）%in group5、or（65.00±0.59）%in group6（P＜0.01），andgroup5showed remarkable decrease on Bcl-2expression and increase on Baxexpression. ConclusionMethyl methacrylate could promote the cardiomyocyte apoptosis.The possiblemechanism is to inhibit the expression of Bcl-2and to promote the expression of Bax.