The Repair Of The Nerve Growth Factor On Nerve Injury And The Structural Reconstruction Experimental And Clinical Studies

objective: To combine the experiment of the rat nerve repair after itssciatic nerve injury with the observation of the efficacy of clinical cases at thesame time in order to get the correlation of the nerve growth factor nerve repairefficacy in animal models and clinical efficacy and provide a useful referenceto the clinical medicine practice. Methods: To study in animal experimentsand clinical trials. In animal experiments, the rats were randomly divided intofour groups as the normal control group, the saline group, the vitamin of B1B12group, and the nerve growth factor group, the rest of the groups except thenormal control group were anesthetized with0.5%sodium pentobarbitalaccording to30mg/kg in abdominal cavity. The four groups were surveyed innerve electrophysiology and neurobehavioral science, then the rat sciatic nerveby light microscopy with HE staining and silver nitrate staining were done.The observed indicators were the rat sciatic nerve conduction velocity and itspathological microscopic morphology. Analysis of variance and analysis ofleast significant difference of SPSS13.0were used in the comparison betweenthe normal group and the intervention group and between the interventiongroups severally. In Clinical trials, the240cases of nervous system damagepatients in the Department of Neurology in the Third People’s Hospital ofChengdu from January to December in2011were regarded as the object of study, plus nerve growth factor intramuscular injection basing on theconventional therapy, including60cases respectively as diabetic peripheralnerve injury, idiopathic facial paralysis, Alzheimer’s disease, Parkinson’sdisease. Chi-square test, independent samples t test and paired t-test inSPSS13.0were conducted in the Clinical trials. Results: In the animalexperiments, the electrophysiological improvement of the nerve growth factorgroup was significantly higher than the other groups; the electrophysiologicalimprovement of the vitamin group was higher than the saline group and thenormal group; the electrophysiological improvement of the saline group andthe normal group was no significant difference. In the rat sciatic nerve biopsy,100%fasciculus continuous, myelin100%complete, no myelin degeneration,interstitial inflammation+and interstitial hemorrhage+was observed in thenormal control group;20%fasciculus continuous, myelin20%complete,myelin80%degeneration, interstitial inflammation++++and interstitialhemorrhage++++was observed in the saline group;50%fasciculuscontinuous, myelin50%complete, myelin50%degeneration, interstitialinflammation+++and interstitial hemorrhage+++was observed in the vitamingroup;90%fasciculus continuous, myelin90%complete, myelin10%degeneration, interstitial inflammation++and interstitial hemorrhage++wasobserved in the nerve growth factor group. In clinical trials, for diabetesimproved in the treatment of peripheral neuropathy using the independentsamples t test, significant difference was observed between the control group and the treatment one; the affected side and the uninjured one improvement ofthe orbicularis oris muscle for idiopathic facial paralysis after treatment usingthe independent samples t-test, the affected side difference between the controlgroup and the treatment one was statistically significant, and the uninjured sidedifference was not statistically significant; for Alzheimer’s diseaseimprovement after treatment using independent samples t-test, differencebetween the control group and the treatment one was statistically significant;for Parkinson’s disease improvement after treatment using independent samplest-test, difference between the control group and the treatment one wasstatistically significant. Conclusion: Observed in pathology, the nervebundles continuous situation of the nerve growth factor group and the myelinintegrity were much better than the vitamin one and the saline one, however,the myelin degeneration and the interstitial inflammation were significantly lessthan the vitamin one and the saline one. After the rat sciatic nerve injury,intervening the nerve growth factor group, the vitamin B1B12one and the salineone respectively by using the analysis of variance and the LSD method forpairwise comparisons, the electrophysiological improvement of the nervegrowth factor group was significantly higher than the other groups, theelectrophysiological improvement of the vitamin group was higher than thesaline one and the normal one, and the electrophysiological improvement of thesaline group and the normal group was no significant difference. The240patients suffering from diabetic peripheral neuropathy, idiopathic facial paralysis, Alzheimer’s disease and Parkinson’s disease were grouped randomlyfor4. In the4groups, the efficacy of the nerve growth factor group wasstatistically significant (P<0.05) compared with the control one, which madeclear that although the basic treatment efficacy was determinate, the addition ofNGF treatment could significantly promote the recovery of the nerve injury.