Relationship Between The Mitochondrial DNA Genetic Variants And Type2Diabetes Mellitus In Yanbian Population

Objective:To investigate the prevalence of mitochondrial DNA (mtDNA) variants in patients with type2diabetes mellitus in yanbian population and to explore the relationship between the mitochondrial DNA genetic variants and type2diabetes mellitus,familial diabetes mellitus.Methods:one hundred and eight healthy controls with normal glucose tolerance,three hundreds and twenty-eight patients with type2diabetes(80patients with family history) and one familial diabetes mellitus individuals in yanbian population were recruited for the study. The variants of mtDNA, including ND1, tRNA, ND4and D-Loop region were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Finally, variants of mtDNA were confirmed by DNA direct sequencing. Biochemical indicators of all individuals were analyzed with SPSS17.0packages.All biochemical indicators data of research subjects were expressed with mean±standard deviation and were analyzed using t test (abnormal distribution data was converted into another), frequence using x2inspection.Analysis revealed statistically significant difference(p<0.05). Results:(1) In the diabetic group, the mt3316G→A variant were detected in12subjects. The variant rates of3316G→A were3.7%; in the control group, the mt3316G→A variant were detected in4subjects, the variant rates of3316G→A were3.8%(x2=0.000,p>0.05)(2.)In the diabetic group, the mt3394T→C variant were detected in24subjects, the variant rates of3394T→C were7.3%; in the control group, the mt3394T→C variant were detected in2subjects, the variant rates of3394T→C were1.9%(x2=4.328, p<0.05).(3) The mitochondrial DNA3243A→G,3593T→C and3714A→G point variants were not detected both in the diabetic patients and the controls.(4) In the diabetic group, the mtDNA12026A→G variant were detected in20subjects (16subjects with diabetic nephropathy), the variant rates of mtDNA12026A→G were6.1%; in the control group, the mtDNA12026A→G variant were detected in1subject, the variant rates of12026A->G were0.9%(x2=3.740, p<0.05).(5) In the diabetic group, the mtDNA16189T→C variants were detected in50subjects, the variant rates of mtDNA16189T→C were15.2%; in the control group, the mtDNA16189T→C variant were detected in10subjects, the variant rates of16189T→C were8.6%(x2=3.216, p>0.05),the frequencies of the variants mentioned above were not statistically different between the diabetic and control groups.(6) In the diabetic group, the variant of mitochondria ND1and D-Loop region have no relation with clinical biochemical data between the variation and nomal groups (p>0.05).(7) Biochemistry data has revealed statistically significant difference about FBG, HbA1C and Cr in T2DM between mtDNA12026A→G variation and nomal groups (p<0.05).(8)In one familial diabetes mellitus.3had mtDNA3394T→C variant,the point variants of other were not detected.Conclusion:(1) Mitochondrial DNA3316G→A,16189T→C variant have no relation with type2diabetes mellitus in yanbian population.(2) The point variant of mitochondrial DNA3394T→C has relation with type2diabetes mellitus and familial diabetes mellitus in yanbian population.(3) The mitochondrial DNA3243A→G,3593T→C and3714A→G point variants were not detected in Yanbian population, imply those variant rates were extremely low.(4) The point variant of mitochondrial DNA12026A→G has relation with type2diabetes mellitus and it can worsen medical conditions such as diabetes.(5) The point variant of mitochondrial DNA12026A->G has relation with FBG,HbA1C and Cr in T2DM group.