Establish The Animal Model Of Nonalcoholic Fatty Liver And Compare The Treatment Effects Of The Two Drugs

Nonalcoholic fatty liver disease （NAFLD） includes a spectrum of hepaticpathology that resembles alcohol-induced liver disease but develops in individualswho are not heavy drinkers. But the exact pathogenesis of NAFLD remains poorlyunderstood which resulted in an lack of effective drugs for NAFLD treatment.Establishing the animal model of NAFLD and Studying on the treatment effects ofpioglitazone and eicosapentenoic acid（EPA）can provide us with a reference forclinical NAFLD therapy.High fat diet is used to establish NAFLD animal model in this study.48rats wererandomly divided into normal control group(NG,12rats) and high fat diet group(36rats), after8weeks of continuous feeding, high fat diet group were divided into highfat control group(HG,12rats), a pioglitazone treatment group(PG,12rats)and a fishoil treatment group(FG,12rats). After6weeks of drug intervention, all rats wereweighed and dissected. Then we weighed all livers and calculated liver index. Contentof the following components were measured: serum ALT, AST, TG and TC of serumand liver homogenate, FBS, FINS, FFAs and MDA of liver homogenate. Then weanalyzed liver pathological section.Results show that the weight, liver index, the contents of serum ALT, AST, TGand TC of serum and liver homogenate, FBS, FINS, FFAs and MDA of liverhomogenate increased significantly in HG rats, which shows an statisticalsignificance comparing with NG(p<0.05). Steatosis with big bubbles andinflammatory cell appeared in pathological section. At the same time, the levels ofthese components in PG and FG were lower than HG, but still higher than NG，whichboth show an statistical significance comparing with NG and HG(p<0.05). Emptybubbles can be seen in pathological section while inflammatory cells infiltration canhardly be observed.In this study, rat NAFLD model has been successful established after8weeksof high fat feeding. PIO and fish oil can improve NAFLD to a certain extent, and theyhave some difference in a number of indicators without apparent difference in theoverall treatment effect. Since PIO can improve the insulin resistance(IR) and reducethe occurrence of inflammation, fish oil can decrease fat content of liver through itsmain functional composition EPA, we predict these may be the mechanisms of the effects of PIO and EPA.