Creation Of Aortic Dissection Model In Canine

Objective:To establish the canine model of Stanford type B aortic dissection through three methods, analyze the merits and demerits of varies methods, approach the unsettled questions from different angles, and provide certain evidence for establishing a more ideal, realistic model in the near future.Methods:Method One:The incision between the third and fourth intercostals spaces of the left chest was used to enter the thoracic cavity. Continuous and everting suture pattern was adopted to suture the artificial blood vessel patch to the descending aortic wall. Finally, created the entry point by needle.Method Two:After thoracotomy was done, the entry and reentry for the aortic dissection was created surgically. Utilize nerve dissector to dissect space which was located in the media. Fogarty arterial embolectomy catheter was then used to dissect and expand’false lumen’ between the two entry points. The distal media and adventitia were sutured to the proximal aorta.Method Three:After thoracotomy was done, the entry for the aortic dissection was created surgically. Utilize nerve dissector to dissect space which was located in the media. Fogarty arterial embolectomy catheter was then used to dissect and expand ’false lumen’ for a distance. Both ends of the distal intimal flap were sutured to the adjacent aorta. The distal media and adventitia were sutured to the proximal aorta. Results:The procedure of Method One was simpler, and the risk was much lower than other two methods. However, the entry cannot maintain long-term patency. The false lumen could easily occluded by thrombus, and there was no separation in the aortic wall. So, it is not very appropriate for studying the natural course of dissection through this method. The model made by Method Two also exist the problems that the entry may not maintain long-term patency and false lumen was occluded by thrombus, alongside with the limited length dissection conducted by Fogarty arterial embolectomy catheter and the complex procedure. The improvement way of suturing of intimal flap in Method Three resulted in blood stream entering the pocket and the propagation of dissection. The imaging method CTA also showed an obvious formation of false lumen. So the Stanford type B aortic dissection animal model could be successfully established via Method Three.Conclusions:1.The canine model of AD which was configuarted via Method Three is the most ideal method that compared with other two methods. Besides, the imaging of CTA of it was similar to human Stanford type B aortic dissection in clinic.2.This experiment laid research foundation and provided reliable experimental basis for establishing the animal model of AD, which should accord with AD pathophysiological change. KEYWORDS:artery, dissection, animal model, canine...