| Objiective:To study the protective effect and investigate possible mechanisms of buflomedil on hepatic ischemia-reperfusion injury (HIRI) in rats.Methods:45adult male rats were divided randomly into the following3groups (15rats each group):Group A, sham operation group; Group B, injected the ischemic reperfusion model with normal saline by the dorsal penile vein at1h before ischemia and after reperfusion; Group C, injected the ischemic reperfusion model with buflomedil (10mg/kg) by the dorsal penile vein at1h before ischemia and after reperfusion. The rats of group B and group C were blocked the left-median liver lobe with a clamp for45minutes. And5rats in each groups were sacrificed at2h、6h and24h post reperfusion. Blood samples were obtained to measure the levels of ALT、AST、IL-1and TNF-a. Liver tissues were collected at the same time to observe histologic changes, the level of TNF-a mRNA was detected by RT-PCR, and the level of the protein of NF-κB p65was measured by Western Blot.Result:1.Compared with Group A, the serum levels of AST, ALT, IL-1and TNF-a in group B and C were significantly increased; pathologic histology tests found out the damage of cells and tissues in group B and C were more serious; the level of TNF-a mRNA in group B and C was increased; and the level of protein of NF-κB p65in caryon were significantly increased (P<0.05).2. Compared with Group B, the serum levels of AST, ALT, IL-1and TNF-a in group C were significantly decreased; pathologic histology tests found out the damage of cells and tissues in group C were alleviated; the level of TNF-a mRNA in group C detected was decreased; and the level of protein of NF-κB p65in caryon were decreased (P<0.05).Conclusion:1. Precondition with buflomedil inhibits the hepatic ischemia-reperfusion injury.2. Protective effect of buflomedil in the ischemic reperfusion model may associate with inhibition of NF-κB activation,which may downregulate the release of IL-1and TNF-α. |
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