P27^kip1 Changes And Apoptosis Of The Vascular Endothelial Cells And Pathology In The Cavernous Venous Malformation Before And After The Copper Needles Retained Treatment

Objective:To investigate the effect of copper needles retained in surface cavernous venous malformation on the expression of factor Ⅷ antigen P27kip1and apoptosis as well as histological and pathological changes in the vascular endothelial cells. To explore the possible mechanisms in treatment in cavernous venous malformation by copper needles retained.Method:①From September2009to January2012,6cases with surface cavernous venous malformation were selected from the Department of Plastic and Cosmetic Surgery, The Third XiangYa Hospital of Central South University. All cases understood and agreed to their treatment (A total of12cases of the comparative samples were selected).②The copper needles with a blunt point and a circularity end, which were made of industrial copper wire with diameter of3mm were sterilized before treatment. All cases were retained12-56copper needles (average30) in the local lesions under anesthesia for1week.③All the samples were dyed in hematoxylin-eosine, after that we observed the histological and pathological changes in the vascular endothelial cells under the microscope of Nikon Eclipse80i. Via immunohistochemical techniques, we observed that the factor Ⅷ associated antigen located in the vascular endothelial cells, and the expression differences of P27kip1in the vascular endothelial cells before and after treatment, while computing the positive ratio. We observed the apoptosis of the vascular endothelial cells via TUNEL method and calculated the apoptosis index. We took pictures by high resolution Nikon DS and NIS-Elements AR3.0software. We used the Ver3.00software for digital image acquisition and the SPSS17.0statistical software for statistical analysis.Results:All the6cases were involved in the result analysis.①After treatment, all the area of the lesions were narrowed compared with before.②Histological and pathological changes:Before treatment, part of blood vessels showed a cystic irregular expansion of its lumen sizes, wall of uneven thickening in the cavernous venous malformation; After treatment, most of the vascular structure were shrank or disappeared, while remaining a large number of lightly stained and unclear connective tissue with a lot of inflammatory cells.③The factor Ⅷ associated antigen was observed as dispersed brownish yellow granules in the cytoplasm of endothelial cells in cavernous venous malformation.④The expression of P27kip1was significantly higher after treatment than before in nucleus of the vascular endothelial cells(P<0.05).⑤Varying degrees of apoptosis were appeared before and after treatment. The level of apoptosis was not significant when compared with before(P>0.05).Conclusion:①After treatment, the size and area of the lesion were reduced. Also, the vascular lumen、vascular endothelial cells and vascular structures were narrowed or disappeared.②The expression of P27kipl was significantly higher after treatment than before in nucleus of the vascular endothelial cells.③Varying degrees of apoptosis were appeared before and after treatment. The level of apoptosis was not significant when compared with before.④Copper needles retained treatment may block the vascular endothelial cell proliferation by up-regulating the expression of P27kip1in vascular endothelial cell nucleus, so as to achieve the purpose of treatment of cavernous venous malformation.