Effect Of Disodium Cantharidinat On Radiosensitivity Of Nasopharyngeal Carcinoma CNE-2Cells

ObjectiveThis experiment aims to investigate the effects of Disodium Cantharidinat on the radiosensitivity of nasopharyngeal carcinoma CNE-2cell line in vitro and its mechanism.MethodsCell growth inhibition was assessed by MTS after incubated with Disodium Cantharidinat at various concentrations and times. nasopharyngeal carcinoma CNE-2cells were divided into4groups:blank group, drug group(deal with Disodium Cantharidinat),irradiation group(deal with irradiation)andcombination group(deal with Disodium Cantharidinat and irradiation).The effects of Disodium Cantharidinat combining radiation to CNE-2cells were tested by clonogenic assay,"multi target single hit survival model" SF=1-(1-e-D/D0)^N was used for dealing with data, then the cell surviving curve, Do, Dq and SER were obtained. When CNE-2cell line was treated with different concentrations of Disodium Cantharidinat for24hours, curcumin and apoptosis ratio was analyzed by Flow cytometry(F-CM).ResultsDisodium Cantharidinat could inhibite the growth of CNE-2glioma cell line, and IC50of48h was320mg/L.Disodium Cantharidinat20mg/L had radiosensitive effect on CNE-2cells exposed for24h, and SER of which was1.43.Irradiation or Disodium Cantharidinat resulted in cell cycle arrest at G2/M phase in CNE-2cells. Apoptosis rates of trial group20mg/L irradiation were(18.84±0.34)%、(5.37±0.4)%、(12.68±0.2)%and control group was(1.82±0.26)%.there was significant difference among groups(P<0.05).Conclution1.Disodium Cantharidinat can inhibit the growth of CNE-2cell line, and the effect was associatted with the concentration and reaction time.2. Disodium Cantharidinat can promote radiosensitivity of CNE-2cell line.3. Radiosensitization by curcumin is possibly associated with the arrest in CNE-2cells following radiation exposure.