| Objctive:To investigate the expression and clinical significance of receptor for advanced glycation end products (RAGE) in peripheral blood from chronic hepatitis B patients.METHODS:ELISA was used to detect the levels of RAGE in serum from50patients with chronic hepatitis B and22healthy volunteers. The expression of RAGE mRNA and NF-κB p65mRNA were detected in peripheral blood mononuclear cells(PBMCs) by reverse transcriptase polymerase chain reaction (RT-PCR). The level of TBIL and PTA were examined by biochemical methods.RESULTS:①RAGE expression occurred in both healthy volunteers and chronic hepatitis B patients;②The levels of RAGE-mRNA in PBMCs were significantly upregulated in severe hepatitis patient group and chronic hepatitis B patient group compared with normal group (P<0.05);③The levels of NF-κB p65-mRNA in peripheral blood mononuclear cells (PBMCs) were significantly upregulated in severe hepatitis patient group and chronic hepatitis B patient group compared with normal group (P<0.05);④The levels of RAGE in serum in severe hepatitis patient group and chronic hepatitis B patient group were higher than that in normal group (P<0.05);⑤There was no correlation between RAGE expression and TBIL in serum (P> 0.05), No correlation between RAGE expression and PTA in serum was observed(P>0.05).⑥There was a positive correlation between RAGE-mRNA expression and TBIL(P<0.05), while negtive correlation between RAGE-mRNA expression and PTA(P<0.05), There was a positive correlation between NF-κB p65-mRNA expression and TBIL(P<0.05), negtive correlation between NF-κB p65-mRNA expression and of PTA(P<0.05), while positive correlation between RAGE-mRNA expression and NF-κB p65-mRNA expression(P<0.05).CONCLUSION:①The level of RAGE in serum was highly expressed in chronic hepatitis B patients;②RAGE-mRNA and NF-κB p65-mRNA expression were upregulated in chronic hepatitis B patients;③RAGE and NF-κB p65may play an important role in the development of chronic hepatitis B. |
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