Changes Of Plasma Vascular NF-kappaB In Patients With Left-to-right Congenital Heart Disease And Pulmonary Artery Hypertension

ObjectiveThis study was designed to test the level of nuclear factor-kappaB(NF-kappaB) of left-to-right shunt congenital heart disease (CHD) with pulmonary arterial hypertension, and to investigate the relationship between NF-kappaB and CHD with pulmonary arterial hypertension.MethodsA total of78patients with left-to-right shunt congenital heart disease were enrolled in the study during August2011to March2012(19of male,59of female). Right heart catheterization were taken in all patients, pulmonary artery systolic pressure (sPAP) and mean pulmonary artery pressure (mPAP) were on the record. The patients were divided into4groups according to the levels of mPAP, including normal PAH group(mPAP≤25mmHg,20patients); mild PH group(25mmHg<mPAP≤35mmHg,21patients); moderate PAH group(35mmHg<mPAP≤45mmHg,14patients) and severe PAH group(MPAP>45mmHg,23patients). We collected pulmonary artery blood for2mL and measured the levels of NF-kappaB by enzyme linked immunosorbent assay (ELISA), and investigated the relationship between NF-kappaB and PAH. All calculations were performed by SPSS version16.0(SPSS, Inc., Chicago, Illinois).Results1. There were significant differences about the Plasma level of NF-kappaB in the four groups,(P<0.01). The level of Plasma NF-kappaB in case of serious PAH>moderate PAH>mild PAH>non-PAH group(P<0.01).2. We also found a positive correlation between the levels of NF-kappaB and mPAP (r=0.856, p<0.01).and the positive correlation between the levels of NF-kappaB and sPAP (r=0.859, p<0.01)ConclusionNF-kappaB participates in the pathological physiology process of left-to-right shunt CHD and PAH, the levels of NF-kappaB may be regarded as one of the markers to evaluate the extent of patients with CHD with PAH.