An Investigation Of Carried Status Of Three Pathogens In Ticks And Mice In Forest Region Of Heilongjiang

Objective:To minitor the infection status of Anaplasma phagocytophilum (AP), Borrelia burgdorferi sensu lato (B.b.s.l) and spotted fever group Rickettsia (SFGR) in ticks and rodents in forest area of Heilongjiang province. Further, to understand the tendency and the distribution of the pathogen in ticks.Methods:Ticks were seized by frag from the area where is30*30square meter in each forest farm; Regular survey was proceeded for the capture of rodents in Da-shigou forest farm and irregular survey was taken in other area. All the sample’s DNA extract and then check out the infectous one by PCR or Nested-PCR, and parts of proved infected samples set to commerce sequencing for corresponding analysis. The whole data we got were finally analyzed by statistics motheds and Geographic Information System.Results:①The research totally catch2535ticks, including1934Ixodes persulcatus,409Haemaphysalis conicinna and10Dermacentor silvarum; the structure of the speices of ticks lived in the three forest farms is different. The dominant ticks in Da-shigou and Er-zhan are Ixodes persulcatus and Haemaphysalis conicinna, and in Hei-niubei lived the two speices above.②No significant difference between the infections of AP in Ixodes persulcatus(4.55%), Haemaphysalis conicinna(3.42%) and Dermacentor silvarum(0.00%), but the difference of the infectious rate of Ixodes persulcatus in Er-zhan was statistically significant (P=0.008).③The difference of the rates of B.b.s.l in ticks mentioned above were significant and the rates were22.39%,4.64%,0.00%. No significant difference among the ticks in different forest farms.④The difference of the rates of SFGR in ticks mentioned above were significant and the rates were8.95%,7.58%,4/10. And the rates of SFGR of Haemaphysalis conicinna in Hei-niubei were higher than the two other forest farms.⑤We seized356rodents and mainly constituted by Apodemus agrarius and Clethrionomys rufocanus. The infectious rates of AP, B.b.s.l and SFGR are0.84%,6.18%and10.11%.⑥Squencing analysis showed there was Gene polymorphism in the three pathogen in our country, and we firstly detect the B.valaisiana andB.burgdorferi genotype of B.b.s.l from rodents in northeast China, andalso check out B.valaisiana-like group genetype in ticks in the same area.⑦Dynamic investigation showed that the major Ixodes persulcatuswere more likely appearance in June or July and the Haemaphysalisconicinna were more likely appearance in early June; The distribution ofthe pathogen in ticks showed the rates of SFGR in Da-shigou and therates of AP and SFGR in Hei-niubei existed the area which is morehigh-risk than other area.Conclusion：①The structure of ticks and rodents in differentforesr farm or region is not the same, as well as the pathogen theycarired.(D It is the first time detected the B.valaisiana-like groupgenetype of B.b.s.l in ticks, and the B.burgdorferi genetype andB.valaisiana genetype of B.b.s.l in rodents.③Dynamic investigationshowed that the peak activity of of tick not only related to their habits,but also closely related to external factors such as environment, climate;Spatial analysis showed that the distribution of some pathogens in ticksindicated that high-irsk area existed and which is the specific factor isneed further reaserch.