The Expression Of Microtubule-related Protein MAP-2in Hippocampus Of Rats In Splenectomy

[Objective]To investigate the expression of Microtubule-related protein MAP-2in hippocampus of rats with cognitive decline after splenectomy.[Methods] Sixty SD aging (22-23months) female rats were randomly divided into three groups:control groups, isoflurane anesthesia groups(A1、A3、A7) and splenectomy groups (S1、S3、S7). The rats of isoflurane anesthesia group were inhaled anesthetic isoflurane (2%isoflurane concentration,95%oxygen concentration) for2hours. Splenectomy groups were accepted spenctomy with isoflurane anesthesia. Rats in control groups (C1, C3, and C7) did not be disposed. Cognitive function was assessed in a reversal-learning version of the Morris water maze (MWM) before and after surgery. Hippocampal MAP-2contents were measured at each time point.[Results] Surgical trauma resulted in impairments in distance and latency only on postoperative day1(P<0.05respectively) in aged rats. Aged rats exhibited impairments on day1(P<0.05) that persisted until postoperative day3(P<0.05). Immunohistochemistry assay further showed the reduction of MAP-2masculine neuron rats in the hippocampus, and the decreased expression of Microtubule-related protein MAP-2in Western blot assay on postoperative day3and7, compared to control groups.[Conclusion]Overall, these findings suggest that surgical trauma, rather than anesthesia, resulted in cognitive function impairment potentiated by aging. The decreased content of MAP-2in Hippocampal might mediate trauma-induced POCD.