| This subject on the basis of review of medical theory in coronary heart disease, theclinical experience of the mentor treatment of coronary heart disease from the heart of thiskidney theory starting combination to establish Bushenhuoxue acupuncture method,intended to emphasize the deficiency and blood stasis in the coronary heart disease theimportance of its efficacy and safety of the treatment of angina pectoris compared with theclinical observation of the system. Meanwhile, the production of myocardial ischemiamodel in rats, cultured bone marrow cells and peripheral blood cells, the use of flowcytometry, immunofluorescence technique, Western blot, and confirmed Bushenhuoxueacupuncture method to mobilize bone marrow EPCs and endothelial repair role for theclinical acupuncture treatment of angina pectoris, a new group hole idea. This clinicalresearch study is divided into two parts of the experimental study, the main researchmethods and results are as follows:Clinical studyObjective To observe the filling kidney invigorate the acupuncture treatment ofcoronary heart disease, angina clinical curative effectMethods Selection stable coronary heart disease, angina patients tired of60cases,random single-blind divided into two groups, and the control group30cases, the treatmentgroup to using the kidney invigorate the acupuncture, acupuncture, midriff with shenshu yu,heart shenshu point primarily acupuncture point; The control group in the hole hole mainlychoose close. Once per day for4weeks.Results The patients and the control group angina pectoris total effective rate was90.00%,63.33%; Kidney blood stasis, heart attack resistance certificate card pulse pointsbefore and after treatment, and patients the total effective rate was86.67%, the comparisongroup total effectiveness60%, its (P <0.05); Before and after treatment symptom scores oftraditional Chinese medicine, Chinese medicine treatment group after symptom scoressignificantly, and slow significantly greater than the control group (P <0.01).Nitroglycerin stop reduction rate, reduce stop group rate93.33%; The control group stoprate reduced80.00%, two groups of comparisons (P <0.05). Electrocardiogram curativeeffect, patients and control group total effectiveness were73.33%,50.00%, two groups ofcomparisons (P <0.05).Conclusion The kidney invigorate the acupuncture treatment of coronary heartdisease, angina has good clinical curative effect, can significantly ease angina, eliminatethe major clinical symptoms, reduce nitroglycerin consumption, improveelectrocardiogram, better than acupuncture point in close. Objective To explore the function and mechanism of the acupuncture methods ofBuShenHuoXue on mobilize the bone marrow EPCs and repair endothelial.Methods The rat ligation of the left anterior descending coronary artery copy ratsmyocardial infarction model, randomly divided into model group (M group), theobservation group (filling kidney invigorate the acupuncture group)(group G) and controlgroup (shut in group)(D group), and the other10only normal rats as blank group (Kgroup), a total of four groups.4weeks after take each rat peripheral blood in the bonemarrow and the separation of EPC training, training7days collecting stick wall cells, useflow cytometric in EPC phenotype appraisal, immune imprinting method (Western blot)test bone marrow matrix metalloproteinases9(MMP-9) expression, in addition the ELISAmethod (ELISA), nitrate reductase, colorimetric method and enzymatic technicalinspection of clear liquid on vascular endothelial growth factor (VEGF), stromal cellsderived factor1-a (SDF-1a), nitric oxide (NO), superoxide dismutase (SOD) change.Results1. The EPC phenotype identification: bone marrow CD34+/CD133+positie, theobservation group (60.4士5.6%) than model group (20.5士5.7%) was significantlyimprove (p <0.01), and significantly higher than those in the control group (28.1士3.4%)(p <0.05); Peripheral blood CD34+/CD133+positie, the observation group (9.5士2.1%)was significantly higher than the other three groups (2.3士1.4、1.1%士0.4%,0.8士0.1),the difference is very significant (p <0.01). The control group, the model group and in the blank group two three more, no significant difference (p>0.05).2. Bone marrow endothelial progenitor cells MMP-9immune imprinting strip analysisshows that, the observation group bone marrow endothelial progenitor cells MMP9produce lower amount of model group increased significantly, and higher than those in thecontrol group (p <0.01); Model group and blank group there was no significant differencein sex (p>0.05).3. Clear liquid VEGF in peripheral blood EPC, SDF-1a testing results show that theobservation group and control group all can improve VEGF, SDF-1a content, and theobservation group higher than those in the control group,(p <0.01). Model group a blankgroup has no significant difference (p>0.05).4. The clear liquid NO peripheral blood EPC, SOD tests showed that, the observationgroup NO and SOD was higher level,(p <0.05), but rose significantly reduce the modelgroup, control group and model group and blank group compared, NO significantdifference (p>0.05).Conclusion The acupuncture methods of BuShenHuoXue can mobilize bonemarrow EPC, peripheral blood to the damaged area and repaired endothelial, give play tothe role of "own transplantation". Its mechanisms may and ischemic heart muscle, VEGFin SDF-1a and down-regulate activate MMP-9signaling pathways involved. |
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