Research On Extraction Purification And Quality Standards Of Active Principles Of Radix Isatidis In Heat-clearing And Detoxicating

Bolting the active principles of Radix Isatidis in heat-clearing and detoxicating, and then studying the extracting and purificating artwork of active principles, finanaly erecting the quality standards. Basing of these work, it is possibily to research the best marching proportion of active principles.Using membrane separation to separate Radix Isatidis into different sections, their anti-inflammatory effects were evaluated by mice models treated by acetic acid-induced celiac capillary permeability and xylene—induced mouse ears.Using macroporous resin and ion exchange resin further separate Radix Isatidis into different sections, and also evaluated by the two mice models, finally, detemining their chemical property by color reactions. Erecting assaying way of total alkaloids, total amino acid, total organic acid and polysaccharide. On the base of mon-factor investigation and orthogonal experiment, the Radix Isatidis was extracted by percolating and then backstreaming.The polysaccharide of Radix Isatidis was purificated by mean of freeze thawing-enzymolysis-membrane separation multi-technique. Researching the cation exchange resin purification artwork of total alkaloids and total amino acid. Researching the purification artwork of total organic acid by mean of anion exchange resin coupling organic solvent abstraction. Finalily, the purity of total alkaloids acid were more than60%,the purity of total amino acid were more than80%, the purity of total organic acid were more than70%, the purity of polysaccharide were more than60%. Eracting quality standards of active principles of Radix Isatidis with TLC, UV and HPLC, especially researching HPLC finger print of total alkaloids and total organic acid of Radix Isatidis. these can assurance the quality of active principle and new Radix Isatidis praeparatum.