The Concentration Analysis Of OPG And RANKL Before And After Hip Replacement Surgery In Peripheral Blood

Background With the artificial joint materials and related operation technology rapiddevelopment，more and more patients have received the artificial jointreplacement.But，the around to the bone dissolve aseptic loosening clinically have notbeen solved after hip arthroplasty and this has become a hip arthroplasty influence toexplore the effect of the important problem. Recent studies show that the artificial jointof aseptic loosening occurrence is related to mechanics factors and biological factorsand biological factors is the main factors. Prosthetic into human body, with jointactivities, will produce the large amount of wear particles. These particles induced thebiological reaction happened, prompting the artificial joint and bone formationboundary between film organization, and with the wear particle and bone cells to absorbstimulating factor increases, cause the world organization of all kinds of film the releaseof bone absorption factor and broken bone cell differentiation and activation, eventuallyleading to the bone dissolve. Mediated periprosthetic bone dissolve cause artificial jointaseptic loosening, OPG and RANKL signal regulating system of the broken bone celldifferentiation, activation and apoptosis plays an important role. In the expression in thehighest OPG bone tissue, OPG have two single body form existence,55KD monomerthrough the two homocysteine (cYS-4oo) between the two key connection and sulfurfoil-assistance system formation, and secreted into exocellular medium, exists inperipheral blood. RANKLmRNA in bone marrow and the expression in the highest, andthe RANKL for a type Ⅱacross the membrane proteins, in body, there are three kindsof existing way, with the cell membrane type soluble molecules form falls off form cutoff, and with soluble molecules form play biological activity, RANKL is also mainlyexists in peripheral blood. After hip arthroplasty, patients in the peripheral blood OPG and the expression level of the RANKL whether changes have not so far from otherdocuments that, in order to understand this problem, this experiment with hipreplacement surgery patients, for example, patients before and after surgery was inperipheral blood and RANKL, OPG the two bone metabolism adjustment factorexpression level change, to evaluate the short term after the body bone metabolism howto regulate, and provide a reference for postoperative forward detection, and to a certainextent to the artificial joint postoperative forward loose in treatment have certainreference value.Objective To measure the concentration of osteoprotegerin (OPG)and receptoractivator of NF—kappa B ligand(sRANKL) in peripheral blood among some patientswho has done hip replacement operation and investigate how our body regulate bonemetabolism in a short time.Methods The OPG and sRANKL concentration of blood serum were measured byELISA, and analyzed the change of expression level of OPG and RANKLResults The OPG concentration of blood serum increase and the RANKLconcentration of blood serum decrease after operation.The ratio of RANKL/OPGdrop.Conclusion Increased expression of OPG compared to RANKL could contribute tothe differential bone formation activity after hip replacement surgery in a short time.