The Expression Of BCL-6in Breast Cancer And Its Significance

Objective: To investigate the expression of BCL-6mRNA and protein in human breastcancer and benign diseases，and to analyse their relationships with clinical pathologicalfeatures in breast cancer patients.Methods: The mRNA and protein expression of BCL-6were detect by in situhybridization and immunohistochemical respectively in127cases breast cancer and50cases benign diseases. In addition, expression of BCL-6mRNA in fresh specimens of32cases breast cancer and28cases benign diseases was detectedby real-time quantitative reverse transcription polymerase chain reaction.Results:1. Compared with benign breast diseases, BCL-6mRNA expression in breast cancerwas significantly increased, there was a significant difference between the two groups(P＜0.05). The expression of BCL-6mRNA was significantly correlatedwith histologic grade (P＜0.05), but not with tumor size, axillary lymph nodemetastasis, TNM stage and age.2. Compared with benign breast diseases, BCL-6protein expression in breast cancerwas significantly increased, there was a significant difference between the two groups(P＜0.05). The expression of BCL-6protein was significantly correlatedwith histologic grade, tumor size, axillary lymph node metastasis, TNM stage andmitotic count (P＜0.05), but not with age.3. There was a positive relationship between BCL-6mRNA expression and its protein expression in breast cancer tissues (r=0.204, P <0.05).4. In fresh breast cancer tissues, BCL-6mRNA expression level was significantlyhigher than benign diseases (P <0.05).Conclusion:1. Compared with benign breast leions, the expression of BCL-6in some breast cancerwas significantly higher.2. The expression of BCL-6in breast cancer was closely related to breast cancerdifferentiation.3. The results implied that post-transcriptional regulatory mechanism may play animportant role in the expression of BCL-6protein in breast cancer.4. BCL-6may be a new valuable molecular marker in breast cancer.