Detection Of Mutations In Nucleot(s)ide-untreated Hepatitis B Virus Infected Patients

Background:Hepatitis B virus (HBV) variants may either emerge in patients with chronic HBV infected patients as a result of positive selection pressure exerted by specific immune response, or during therapy with nucleostide analogues. Naturally occurring HBV variants with primary antiviral resistance are rarely observed.But some reports show that patients with chronic HBV infected patients are natural resistance to nucleostide analogues.Aim:The aim of this study was to use2different methods to analyze HBV variants with natural resistance to nucleostide analogues currently used as therapy for chronic HBV infected patients.Methods:A total of104cases (42cases in group A of Pyrosequencing and62cases in group B of fluorescent PCR)used different methods for detection and did not receive the treatment of nucleos(t)ide analogues ever.Their serum mutations were detected by microcosmic nucleic acid and cross-nucleic acid quantitative determination,Results:Among42patients in group A without receiving the treatment of nucleos(t)ide analogues, spontaneous HBV variants were detected in3subjects(2181mutants and1173mutant). Among62patients in group B spontaneous HBV variants were detected in6subjects(2207mutants,2213mutants,1204mutant and1238mutant).HBV variants account for7.14%、9.68%in each group. Gender, the state of disease, age, ALT, AST, serum HBV-DNA load were not associated with spontaneous HBV mutation.Conclusion:Spontaneous HBV variants (primary drug resistance mutation and compensatory mutation)exist in chronic hepatitis B patients. But the frequence of these variants are low and the meaning of these still need to be investigated.