The Investigation Of Infection And Resistance Mechanism Of Pseudomonas Aeruginosa In Chronic Suppurative Otitis Media

Objective: To investigate the distribution of Pseudomonas aeruginosa in chronicsuppurative otitis media, drug-susceptible and antibiotic resistance mechanism, andprovide preliminary clinical evidence for diagnosis and treatment of chronicsuppurative otitis media.Methods: To collect240cases of patients with chronic otitis media surgery,intraoperative, postoperative lesions of secretion, preoperative and postoperativespecimens were collected for the external auditory canal, intraoperative specimenstympanic sinus/intratympanic acquisition, respectively, to the cultivation ofanaerobic bacteria and aerobic bacteria identification and antibiotic susceptibilitytesting, screening of quinolone-resistant strains; sequence analysis and polymerasechain reaction (PCR)-section83of the detection of Pseudomonas aeruginosa eatbacteria gyrA gene point mutation at codon to explorethe point mutations in thefluorine wow Snow ketones antibiotic resistance of Pseudomonas aeruginosa.Result： Preoperative, intraoperative and postoperative total of240specimens,Pseudomonas aeruginosa culture was positive in38(15.83%of the total detection rate;38/240), preoperative external auditory canal culture was positive in38cases(detection rate of100.00%38/38), tympanic sinus surgery/tympanic culture waspositive in30cases (78.95%detection rate,30/38), postoperative external auditorycanal culture positive for27cases (71.05%detection rate,27/38). SPSS13.0softwarestatistical analysis: the external auditory canal and surgery in the preoperativetympanic sinus/tympanic detection rates were significantly different (P=0.008<0.05, Fisher exact probability method), the tympanic sinus surgery/tympanic andpostoperative external auditory canal no significant difference in detection rate(Pearsonχ2=1.510, P=0.219>0.05);24ciprofloxacin-resistant bacteria, resistancerate of63.16%: ciprofloxacin Pseudomonas aeruginosa, sensitive test for detection of display The Aeromonas MIC>4μg/ml that bacteria is resistant; Sequence analysisshowed that fluoroquinolone-sensitive P. aeruginosa gyrA sequence in GenBank,21different ciprofloxacin resistance of Pseudomonas aeruginosa gyrA gene sequenceoccurred following83password-bit mutation: Thr (ACC)â†'Ile (ATC).Conclusion1. Chronic suppurative otitis media to aerobic infection, Pseudomonas aeruginosahigher detection rate of the total seizure (15.83%;38/240).2.83codon of the P. aeruginosa chromosome DNAgyrA gene mutation is animportant mechanism of resistance fluorine twitter promise ketones antibiotics.