Establish And Effect Evaluation Of Pathological Aggression Animal Model

OBJECTIVETo establish the preferable and repeatable pathological aggressionanimal model what similar with human beings changing in pathology andbiochemestry.METHODS1.To establish of rat model on pathological aggression.40of maleWistar rats, and it is divided into three groups at random:(1)normalaggresssion model: resident-intruder.(2)pathological agression model:①frustration test②non-reward and instigation.(3) control group: normalreal.2.The effect of evaluation of the rat model, it conclude behavior testand biological test.2.1Behavior test2.11Total aggressive times test:①attack bites②m ounting③andoffensive threat of the opponent, the total aggressive times figured up:①+②+③2.12Pathological aggressive behavioral test:①a ttacktoward vulnerable-body regions②persistence attack after intruder displayssubmissive③and attack latency.2.13Other behavioral test:①O pen-Field Test②Elevated plus mazeTest③Sucrose preference test④Olfactory test.2.2Biological test: immunohistochemistry detect the expression of5-HT and c-fos on prefrontal cortex、 hypothalamus and amygdaloidnucleus.RESULTS1.Behavioral test:①Compared with normal aggression（52.5±5.36）and control group（8.83±1.34）in total aggressive times, the pathologicalaggression group（101.17±2.85）increased significantly (P<0.01)；②Somebehavior items of each model group were moderately or highly correlatedto total score (r=0.379～0.929)；③There was a significant differencebetween pathological aggression group and normal aggression group in thenumbers of attack toward vulnerable-body regions, persistence attack afterintruder displays submissive and high attack/threat ratios (P<0.01)；④Thepathological aggression group did not show depression, anxiety andolfactory disorder, except space cognitive function（P＞0.05）. However,the normal aggression group displayed obvious depressive mood.2.Biological test:①In pathological aggressive group, the expressionof5-HT in prefrontal cortex、hypothalamus and amygdaloid nucleus weresignificantly lower than control group [PFC(11.7±0.84); HA(10.1±1.11); AMD(12.5±0.72);(P<0.01)].②I n normol aggression group,theexpressionof5-HT in prefrontal cortex、hypothalamus were significantly higher thancontrol group [PFC(20.9±1.08); HA(19.1±1.77); AMD(22.5±2.08);(P<0.05)], but in amygdaloid nucleus, it had no significant different betweennormol aggression group and control group（P>0.05).③In pathologicalaggressive group, the expression of c-fos in prefrontal cortex、hypothalamus and amygdaloid nucleus were significantly higher thancontrol group [PFC(32.2±3.45); HA(21.7±2.58); AMD(23.7±0.68);(P<0.01)].④In normol aggression group, the expression of c-fos in prefrontalcortex、 hypothalamus were significantly higher than control group[PFC(18.8±2.07); HA(10.7±1.01); AMD(15.4±0.85);(P<0.05)], exceptin amygdaloid nucleus,it had no significant different between normolaggression group and control group（P>0.05).CONCLUSION1. Each behavioral index matches the criteria of pathological aggressionmodel.2. Each biological also matches the criteria of pathological aggressionmodel.3. Meanwhile, it also excludes other factors of disturbing the specificityof the model.4. It suggests this model may be stable and repeatable pathologicalaggression animal model,which may serve as study of the mechnism of pathological aggression.