Research About The Impact Of The Damage On Adult Mouse Embryonic Gene Tbx18

Background:The cardiovascular system disease has become a seriousthreat to human health in modern society, and it is the major cause fordeath,at present in clinical, the morbidity and mortality of myocardialinfarction are very high,so the study of the pathogenesis of myocardialinfarction and developing new therapies for myocardial infarction can notwait.A number of preclinical studies have attempted to repair damagedmyocardium by transplantation of exogenous cells.However，the mainproblem is divided on whether the improvement of cardiac functioncorrelates to long-term benefit and whether this improvement is restricted tocertain subcategories of patients,currently,little is known for people. Inrecent years, the concept of using stem or progenitor cells as thebasis for cardiovascular therapy has gained considerable interest.Objective: To study the impact of myocardial infarction injury on theadult mouse cardiac transcription factor Tbx18expression.Methods: We used the right lateral position by transverse incisionbetween the left3,4rib ligation of the left anterior descending coronarymethods to establish the adult mouse myocardial infarction model,through detecting cTnT within postoperative24hours by the troponin kit,pathological examinating of myocardial infarction tissue sections of7daysafter operation to prove the success of the model, by quantitative PCR wedetected the Tbx18mRNA expression trends in infarcted area at differentdays after myocardial infarction,by immunofluorescence staining we locatedthe expression of the embryonic gene Tbx18after myocardial Infarction,byWestern Blot we detected the Tbx18protein expression after myocardialinfarction.Results:We have successfully established adult mice myocardialinfarction injury model through the right lateral position by transverseincision between the left3,4rib,the survival and accuracy are significantlyhigher than the traditional supine left parasternal vertical incisionmethod,the positive result of cTnT within24hours prompted the initialsuccess of modeling, the Masson results of7days after myocardialinfarction indicated that the infarcted area and the surrounding area had largenumber of collagen deposition and fibrosis, and ultimately determined themodel was successfully established.The quantitative PCR results at differentdays of the myocardial infarction samples in infarcted area suggested thatmyocardial infarction damage could activate the expression of Tbx18, theTbx18expressed highest in infarcted area at3days after the operation,andthen the level gradually decreased,at14days the expression was thelowest.The immunofluorescence results suggested that at7days of the adult mouse myocardial infarction the Tbx18expressed highly in the infarctedarea and the surrounding area as compared to the sham operation group.TheWestern Blot results prompted that Tbx18protein expressed obviously aftermyocardial infarction.Conclusion:Through the right lateral position by transverse incisionbetween the left3,4rib we can successfully establish adult mice myocardialinfarction injury model,and under myocardial infarction conditions, theembryonic gene Tbx18can be activated.