| Background：Depression is thought to be mainly depressive mood in manifestation,which always refers to a mood disorder. At present, depressive disorder hasbeen significant problem worldwide, due to its disability and mortality,bringing tremendous burden to the families and society, whereby theunderlying mechanism is not clear yet. Based on the overwhelmingresearches on structure and function of brain, it turns out to be abnormalityof dysfunction of prefrontal. As report of autopsy on depression patientshowed: there presented that Perfusion of prefrontal, decreasing of thecortex of the anterior cingulate, dwindling volume of neuron as well as lostof glial cells.Proteomics technology is mainly to study on levels of protein expression,amino acid sequence, post-translational processing and protein interactions,at proteinous level, try to understand various functions, physical andchemical or pathological process in cell. And also, the techniques provide apowerful tool to investigate the pathogenesis underlying the mentaldiseases. Hereby, focusing the changes of the CUMS model via Proteomic Techs,which unravels the existing molecular variation in the prefrontal,furthermore, we discussed the underlying mechanism with disease, whichprovides novel idea about the diagnosis and therapy.18healthy adult SDRats were randomly divided into CUMS and control group, after a periodof28days of chronic stimulation on the CUMS group, we conducted theevaluation of sucrose consumption tests and open field tests among all theRats. Afterwards, we utilized the two-dimensional gel electrophoresisrandomly to test3rats, consecutively, identify the differential protein spotsusing matrix-assisted laser desorption ionization time of flight massspectrometry (MALD-TOF/TOF) as well as informatics analysis on threespots. Additionally, to rule out12SD rats (6/each group), focusing thespots in the prefrontal to employ further validation by means of WesternBlot (WB).Results：1. Weight changed on CUMS rats, wilderness experience level andvertical score and sugar preferences all aspects of normal rats werestatistically significant different, which indicated the rat model ofdepression was constructed successfully. In CUMS group, the ratsrepresent statistical significance at scores of vertical and local motor inopen field test and sucrose preferences which uncover the successful modelconstruction as well. We constructed optimizing high-quality, stable, highly repetitive map based on2-D gel electrophoresis, erecting the basis forfurther investigation on the differential protein of rat’s prefrontal withdepression.2. By means of two-dimensional electrophoresis found thatdepression in rat olfactory bulb,60protein spots were differentiallyexpressed, the final identification of31proteins.3. We found60differentialprotein spots in Olfactory Bulb (OB) using2-D gel strategy, and ultimatelyidentified31out of60differential proteins.4. Furthermore, as theBioinformatics analysis of these differential protein spots disclosed theimplication of apoptosis, energy metabolism, and neurogenesis.5. As resultsof Western Blot(WB) shows significant increasing ofDihydropyrimidinase （t=4.094，P=0.002）and milk acid glutathione lyase（t=2.965，P=0.014），whichisinaccordancewith2-DGel results. Whileannexin A3presents no significance between2groups（t=0.169，P=0.869）.Conclusion：1. Chronic stress may lead the related proteins modified onneurogensis and apoptosis in the prefrontal, which infer that theseproteins had close relationship with the pathogenesis under diseasecircumstance.2. Dihydropyrimidinase related protein expressed in theprefrontal and milk acid glutathione lyase2might play a crucial role inthe pathogenesis of depression. |
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