The Research Of The Impact Of Restraint Stress In Peri-implantation For Embryo Implantation And Embryo Development Of Mices And Its Relationship With The Balance Of Th1/Th2

Objective:The aim of the study was to observe the effect of restraint stress on peri-implantation mice blastocyst implantation and embryonic development; meanwhile,the cytokine IL-10and TNF-α mRNA of the uterine decidual tissue were alsodetected after the mice suffered from restraint stress. The present study willinvestigate whether restraint stress affects embryo implantation via Th1/Th2balance.Methods:1. During the peri-implantation period (pregnancy D3, D4, D5), pregnant micesuffered from restraint stress for2hours in order to establish stress animal model.2. The number of implantation site of the pregnant mice on day5was observedby tail vein injection of trypan blue.3. Enzyme-linked immunosorbent assay (ELISA) was used to detectcorticosterone, prolactin and progesterone in peripheral blood of the pregnant mice onday5.4. HE staining was utilized to observe the change of stromal cells and glands inthe uterine decidual tissue.5. RT-PCR assay was used to investigate the mRNA levels of IL-10and TNF-αin the uterine decidual T lymphocytes of the pregnant mice on day5.Results:1. Compared with the control group, restraint stress significantly increasedcorticosterone level in peripheral blood of the pregnant mice on day5byEnzyme-linked immunosorbent assay (P <0.05); while dramatically decreased theprolactin and progesterone levels (P <0.05).2. The number of implantation site of the stressed group was lower than thecontrol group by tail vein injection of trypan blue.3. Compared the control group, the development of the stromal cells and ulterineglands in the uterine of the restraint stress group was significantly inhibited. 4. Blastocyst of pregnant mice on day8was almost the same size and distributedsymmetrically in the control group; while the blastocyst of the restraint stress groupwas smaller and distributed asymmetrically.5. Compared with the control group, restraint stress significantly decreased themRNA levels of IL-10and TNF-α in the uterine decidual tissue (P <0.05); while therelative mRNA level of TNF-α/IL-10of the restraint stress group was higher thanthat of the control group.Conclusions:1. Restraint stress could significantly increase the corticosterone level inperipheral blood of the pregnant mice and decrease the levels of prolactin andprogesterone on pregnant5day.2. Restraint stress dramatically decreased the number of embryo implantationsite.3. On pregnant5day restraint stress significantly reduced the mRNA levels ofIL-10and TNF-α in the uterine decidual tissue; while the relative mRNA level ofTNF-α/IL-10(Th1/Th2) of the restraint stress group was higher than that of thecontrol group, indicating that restraint stress broke the Th1/Th2balance andresponsed towards Th1immune response and affected embryo implantation.