Effect Of Taurine On The Protein Expression Of Key Enzyme And Regulatory Factors Involved In The Process Of Fat Metabolism In Rats With Alcoholic Fatty Liver Disease

Alcoholic fatty liver (AFL) is caused by lipid accumulation in liver cells5％more thanliver weight．It is the most common and most early periods in the development of alcoholicliver disease (ALD). The incidence of AFL increased significantly all over the world andseriously damaged the health of people.Taurine has various biologic effects such as maintaining the normal structures andfunctions of hematological system, immune system,genital system,visual system,cardiovascular system and nervous system. Recently, it has been reported that taurine couldreduce the hepatic injury caused by CCL4,however there are few information about the effectsof taurine on ALD.Alcohol and pyrazole were administered to Wistar male rats together with high fat diet toinduce AFL animal model. Rats were killed, then blood and liver were collected forbiochemical analysis and histological observation. At the same time, protein expression ofPPARα,ACC,CPT-1,FAS,HMG-CoA,SREBP-1in the liver were detected using the methodsof Western Blot.The results showed that serum ALT, AST in AFL model group were significant higherthan control group (P<0.05); the body weight were decreased, while liver index significantlyincreased. The pathological sections of the rats showed significant fatty degeneration of AFLrats.The results of western blot showed that the protein expression ofACC,FAS,HMG-COA,SREBP-1in liver of AFL model group were significant higher thancontrol group, while the protein expression of PPARα,CPT-1were significant lower thannormal rats (P<0.05); taurine administered preventively could decrease the protein expressionof ACC,FAS,HMG-COA,SREBP-1, increase the protein expression of PPARα,CPT-1. Incurative experiment, the protein expression of ACC,FAS,HMG-COA,SREBP-1in taurinecurative group were significant lower than the control group, while the protein expression ofPPARα,CPT-1was significant higher (P<0.05), there were no obvious differences betweentaurine curative groups and control group (P>0.05).All the results indicated the liver could be significantly damaged by12weeks of alcoholand pyrazole administration，AFL rats showed significant fatty degeneration of liver, theserum ALT, AST were increased.The results of western blot indicated that taurine couldinhibit the synthesis of TC by way of up regulation CPT-1protein expression, downregulation of ACC,FAS,HMG-COA,SREBP-1protein expression and promoting the fatty acidoxidation. Meanwhile, taurine could also indirectly inhibit TC biosynthesis by way of upregulation of PPARα protein expression. In this way, it could induce the enzymes related tolipid metabolism, promote β-oxidation and ω oxidation. And taurine may regulate hepatic fatmetabolism.