Evaluation Of The Joint Toxicity Of Pesticide Mixtures And Influence Of The Mice’ Cholinesterase Activities In Whole Blood

Objective:Investigate the joint toxicity of dimehypo and metolcarb; and influence of the mice’Cholinesterase activities. to the science for both mixed, provide a reference to identify potential hazards.Methods:In Kunming mice were clean for experimental animals, according to the experimental design for metolcarb dimehypo and joint toxicity experiments, Is divided into four steps,(1) Dimehypo single exposure LD50test;(2) Metolcarb single exposure LD50test;(3) Dimehypo, metolcarb mixed test;(4) Metolcarb and dimehypo mixed23factorial design test. The exposed group were exposed to the appropriate program to0.1ml/10g exposure by gavage, observed the performance of poisoning animals after exposure, and record symptoms and death, use spss13.0software probit modules that the unit to LD50> Then determined blood cholinesterase activity by hydroxylamine hydrochloride—ferric chloride colorimetric method. Detection of mouse blood AChE on two kinds of pesticide sensitivity, based on the detection of two kinds of pesticide mixture on AChE joint action, through the Bliss method, Mansour method, Sun method, Finney method in the evaluation of the combined effects of mixture.Then using a2x3factorial analysis of variance inspects both on mouse blood cholinesterase impact of the existence of interaction.Results:1. The experimental results of dimehypo LD50for152.15mg/kg, metolcarb LD50for365.47mg/kg, dimehypo, metolcarb1:1mixture LD50for289.24mg/kg;2. Bliss method to get the synergistic effect of (Me-Mt)=21.6%, Mansour method to get the synergistic toxicity was cf=-17.87, Sun method to get the co-toxicity coefficient (CTC)=74.27, Finney method was out of the efficiency coefficient=1.49, the evaluation results are expressed as additive effects;3.Dimehypo single dose of each exposure group the whole blood cholinesterase activity was lower than the normal group, the difference was statistically significant (P<0.05), the lowest rate of3.00%cholinesterase inhibition, the maximum35.19%; Metolcarb single dose of each exposure group the whole blood cholinesterase activity was lower than the normal group, the difference was statistically significant (P<0.01), the lowest rate of12.96%cholinesterase inhibition, the maximum42.22%; Metolcarb dimehypo1:1mixture with all the mice exposed to whole blood cholinesterase activity was also lower than the normal group (P<0.05), the lowest rate of15.12%cholinesterase inhibition, the maximum45.75%;4.Metolcarb and dimehypo mixed23factorial design experiments revealed two effects on mouse cholinesterase Interactions, can be considered dimehypo100%LD50dose and metolcarb100%LD50dose combinations, the most toxic in mice, whole blood cholinesterase inhibition in mice the strongest.Conclusion:1. Metolcarb and dimehypo belong to poisoning pesticide, whole blood cholinesterase activity in mice have different degrees of inhibition;2. This topic should adopt the Bliss method and Mansour method to evaluate mixture combined toxicity.3.Mixed inhibition of cholinesterase activity are more obvious,23factorial design experiments revealed the existence of both the interaction of mouse cholinesterase actiity.