| Objective: To investigate the long-term effects of heme oxygenase1overexpressionon post-infarcted heart function in diabetic rats and its possible mechanism in animalmodel of diabetic myocardial infarction. To provide a new idea on the prevention andtreatment of post-infarcted heart function.Methods: Sixty adult male Wistar rats (350-400g) were randomly divided intosham operation group (sham), diabetes+sham operation group (DM+sham), diabetes+myocardial infarction(MI) group (DM+MI), diabetes+MI+tin porphyrin (SnMP)group (DM+MI+SnMP), diabetes+MI+cobalt original porphyrin (CoPP) group(DM+MI+CoPP). The echocardiography and hemodynamic examination were usedto detect the heart structure and function, and long-term influence of HO-1inducer(cobalt protoporphyrin, CoPP) and activity of HO inhibitor (tin porphyrin, SNMP).Blood Glucose (GLU), bilirubin(TB), blood lipids (TC), C-reactive protein (CRP),serum creatinine (Cr), aminotransferase (ALT) and other indicators were measured.Interleukin-6(IL-6), tumor necrosis factor (TNF-α), nitric oxide (NO), prostacyclin(PGI2), adiponectin, and high sensitive CRP (HsCRP) levels were tested by ELISAmethod.Results: Compare with DM+MI group, the bilirubin, NO, PGI2, adiponectinlevels were increased by overexpression of HO-1, while, the blood glucose, Hs-CRP,IL-6and TNF-α levels were decreased in DM+MI+Copp group. And the LVSP,LVEDP,±dp/dt max were improved, LVEF and LVFS were increased byoverexpression of HO-1in DM+MI+Copp group. The above benefited effects werereversed by administration of SnMP.Conclusion: The long-term heart structure and function were improved byupregulation of HO-1via inhibiting inflammation, improving endothelial function and lowering blood glucose and so on in an animal model of diabetic myocardial infarction. |
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