| Objective:Irritable Bowel Syndrome (IBS) is one of the common functionalgestrointestinal disease, to see diarrhea-predominant irritable bowel syndrome(IBS-D) more. Chinese medicine treatment plays an important role in thisdisease.especially in Sijundishao decoction which is created by my tutor. Inour study,we established the model of IBS-D of spleen deficiency to observethe expression of IL-1β、5-HT、SP、VIP,and try to expore the mechanism ofIBS-D spleen deficiency.Method:After5days to adapt,healthy100male Wistar rats were used in thisstudy. Rats randomly divided into5groups: Normal grou（pN）, Modle group（M）, Control group (C),heigh dose of Sijundishao Decoction(HD), lowdose of Sijundishao Decoction(LD). Normal group wast reated as normals.Theothers are given senna with concentration of0.3g/mL.d-1by gavaging for21days.During the process,we observed the condition of rats stool.After that，made local colon tissue pathology: each group of random take2mouse, To beput to death in immediately after laparotomy materials, take a colonorganization to physiological saline cleaning bowel content, in10%neutralformaldehyde after24hours fixed routine dehydration, paraffin, HE dyed, todo the pathological examination to exclude the intestinal mucosa inflammat--ory lesions.After that the model group was given saline by gavaging; thecontrol group was given pinaverium bromide(1.5mg/ml)20ml/kg; HD groupwas given Sijundishao decoction according to30g crude drug dose/kg-1.d-1;LD group according to15g crude drug dose/kg-1.d-1.Two weeks later, theanimals were fasted for24hours and executed for blood that go through rapidcentrifugation. Conservation of samples of serum, and plasma samples to beused for SP、5-HT、IL-1β、VIP. The expression of SP、5-HT、IL-1β、VIPwere detected by immunohistochemical method.The data was statisticed by SPSS13.0，the date use Χ±s to express.Result：1General conditon: Compared with the control group, the model rats beganto loose stools, listlessness, a coat of dry engage from the third day.Until twoweeks,the modeling was successed. After treatment,HD group wassignificantly better than the other two groups in the term of stopping diarrheaand improving body weight.2Pathological changes of colon:there is no ciginificent changes of colon.Eachgroup has no obvious ulcers, bleeding, erosion, and inflammatory cellinfiltration. In a word,there is no significant differences among the groups.3The results of Serum IL-1β, serum5-HT, plasma SP；3.1Serum IL-1β: compared with other groups, the content of serum IL-1β wassignificantly higher in model group (P<0.05),and lower in normal group(P<0.05).HD group was obviously superior to the control group and LD groupin reducing the expression of IL-1β(P<0.05)；3.2Serum5-HT: compared with other groups, the content of serum5-HT wassignificantly higher in model group (P<0.05),and lower in normal group(P<0.05). Control group were obviously superior to LD group in reducing theexpression of5-HT (P<0.05),but HD group was not；3.3Plasma SP: compared with other groups, the content of serum SP wassignificantly higher in model group (P<0.05),and there no significantlydifference in normal group and HD group(P>0.05).HD group was obviouslysuperior to the control group and LD group in reducing the expression ofSP(P<0.05).3.4Plasma VIP: compared with other groups, the content of VIP wassignificantly higher in model group (P<0.05),and lower in normal group(P<0.05).HD group was obviously superior to the control group and LD groupin reducing the expression of VIP(P<0.05).Conclusion：Sijundishao decoction could improve the general conditon ofthe spleen deficiency type of IBS-D rats.The mechanism is related to regulatethe secretion and release of gastrointestinal hormone like5-HT、SP、IL-1β、 VIP，then play anti-inflammatory antibacterial, adjust the gastrointesti--nal tract movement, enhance bowel mucosal immunity, reduce the intestinalsensitivity, restrain bowel movement, abnormal secretion and abnormalbrain-the role of the bowel nerve conduction, To achieve the purpose oftreatment IBS-D. |
PDF Full Text Request