| [Objective] In the small intestine ischemia reperfusion (intestinal ischemia-reperfusion injury, â…¡/R) based on the model, the juvenile rats and adult rats after ischemia reperfusion of intestine slices HE staining were observed, in juvenile and adult rats during intestinal ischemia-reperfusion injury of intestine morphology when compared, for clinical experiment bedding and guidance.[Methods] By4weeks of experimental animal healthy SD rats aged20, body weight150-200g,10weeks of age (health reference" Laboratory Animal Science"2006,3,1edition. China Agricultural University press,20) rats, body weight250-300g, purchased from Kunming Medical University, both in experimental animal before conventional feeding, preoperative prepare for:drinking water restrictions, fasting12h. Take the rats were divided into young rats (4weeks old) and adult rats (10weeks old) in two groups, each group of20, group of four observation points, each point of observation to5mice, four observation points:at the start of peritoneal free (open only the superior mesenteric artery, no other treatment put to death), ischemia (1H free superior mesenteric artery ligation afterlwhen executed),1H reperfusion (free of superior mesenteric artery ligation for1hours, openl hours),2h reperfusion (free of superior mesenteric artery ligation for1hours, open2hours). Two groups were in the beginning, ischemia, reperfusion, the lh lh2h reperfusion kill the mouse, at a distance from the ileocecal5cm loops based on specimens removed, flushing, fixed, making the wax block standby, the specimens were stained with HE, were observed under light microscope and Chiu’s6scoring, statistical analysis, two groups were few compared with t test, P<0.05indicates that the result was statistically significant.[Results]1juvenile rats group at the beginning of the only free superior me se nt e ric ar te ry w it h o u t c li p p in g, in t e st i n al ti s s u e s of goo d b lo o d su pp ly, in te st i n a lw a ll w it h ou t h y p er e m i a, e de m a, b le e d in g an d u lc e r, no o b v io u s in f la m m a to ry ex ud at e.1sm a ll in te st i n a l w a l l is c h e mi a w h e n p ale, a sm a ll a mo u n t o f in f la mm a to r y ex ud a te.Re pe r f u si o n of in te s t i ne o bv io us c o n ge st i o n, ede ma, a c r o ss th e in te st i n a l w all sh o wedth e da r k r e d, a n d e v e n so me c ya no se d, in te st i n a l b le e d in g a n d u lc e r, b ow el ti s s u ec o ul d be o b se rve d si g n i f ic a n tl y mo r e in f la mm a to r y e xu da te.A d u lt r a t gro u p a t th e b e g in n in g is on ly fr e e me s e nt e r ic a r te r y w it h o u t cli p p in g,in t e st i n a l ti s s u e r e va s c u l a r iz a ti o n is go od, th e in te st i n a l w a ll sm o o th, n o bl e ed in g a n de x ud a ti o n; is c he mi a in a d ul t r a ts c o mp a r ed to th e you n g g r ou p gro u p s in te st i n e co lo rd e e p, n o in f la mm a to r y e x u da te; r e p e r f us i o n in j u r y i n a d u lt r a t gro u p s in te st i n e co lo rd a r k pu rp le, a ls o t h e r e is bl e e di n g a n d u lc er su r face, c o m p a r e d to th e yo un g g ro u p is le ss, an d th e b l e e d i n g p o in t mo r e sc att e r ed, le ss b l e e d i n g, b le e d in g su r f ace mi x edin f la mm a to r y e x ud a t e.2. g en e r al H-E st a i n i n g af t er (1) o n l y f re e su p e ri or me s e n t er i c ar t er y wi t h o ut cl i pp i ng pu p s an d ad u l t r at sm al l i nt e s t i n e mu c o sa l v i l l o u s t om e n t os e mar gin, s t ru c t u r a l i n t eg r i t y, cl ea r, i nt es t i na lv i ll us ep i t he l i al co l u m na r, ne at l y ar r an g ed， CH IU’ s le v e l6s c or e s w e r e0.282±0.10ã€0.28±0.06.(2) i sc h em ia1H (i.e. wi t ho u t i nt es t i na l bl oo d re p er fu s i on) j u v en i le r at s gr ou p v il lou s st ru c t ur e i s st i l l i nt a ct, l e si o n s a r e r e la ti v e ly li g ht, n o b le e d in g, la mi n a pr op r ia e d e ma,e pi t h e li a l g ap is w id e r, c o r te x w it h la mi n a p r o p ria m o de r a te se p a r a ti o n, o ccas i o n all y f e a tu r es to p b r e a ka g e，CH IU ’ s le ve l6s c o r e s w e re2.39±0.43; in a du lt r a ts gro u p v il l o u sst r u ctu r e in te gri t y, le si on s th a n ju ve n il e rat gr o u p l i ght, e p it h e li a l ga p e xp an si o n is le ss, o n th e cor te x w i t h la mi n a p r op r ia h a s a m i l d se p a r a ti o n. Ra r e v il l o us ti p b r e a ka g e， CH IU ’s le ve l6sc o r e s w e r e2.33±0.48.(3) a f t e r r e p e rf us i o n f o r1h, ju v e n il e r a t g r o u p of p a th o lo g y c ha n ge o b vi o u sl y, in t e st i n a l e pi t h e li a l st r uc tu r e is c o mp l e te,sm a ll in te st i n a l v i l l u s e p it h e li a l c e ll sh e d di n g, v i l l ou s da ma ge, la mi n a p r o p ria cell c o mp o n en ts in c r e a s ed, c a p il l a r y e xp o su r e, o ccas i o n a l l y in he r e n t la y er d a ma g e，CH I U’ s le v el6s c o r e s w e r e4.49±032; in ad ul t r a t s g r o u p of p a th o lo g y cha n g eo b v io u sl y, bu t th e r e i s n o ju v e ni l e r ats gro u p. Vi l l u s e pi t h e li a l c e ll sh e dd in g le ss,v il l o u s ti p b r e ak a g e, c a p il l a r y e xp o su r e， CH IU’ s l e v e l6sc o r e s w e r e3.80±0.42.(4)r e p e r f u si o n gro u p2H j u v e ni l e r a t sm a ll in te st i n e s t r uc tu r e g r o up st i l l n ot r eco v er ed, the lamina propria of destruction does not see more, lamina propria capillary exposure, CHIU’s level6scores were4.01±0.14; group of adult rats and juvenile rat groups compared, lesions are relatively light the intrinsic layer, less damage, villous tip breakage light, CHIU’s level6scores were3.98±0.13. The statistics group by paired t test, group to take a single element variance test. P<0.05, showing statistically significant.[Conclusions] Intestinal ischemia reperfusion injury caused by intestinal pathological changes of alignment, juvenile rats with reperfusion injury is heavy, and occurred earlier, early already peaked; adult rat injury is relatively light, slow development. |
PDF Full Text Request