| Objective:To investigate the effects of modified Kuikeling on IGF-1、TGF-β1and CTGF in colitis model rats, and to explore the possible mechanisms of modified Kuikeling on the intestinal fibrosis in the treatment of IBD.Methods:A total of40Sprague-Dawley rats were randomly divided into four groups which were control group(D group), model group(M group), kuikeling group(Y group) and modified Kuikeling group(J group),respectively. Colitis model was induced by trinitrobenzene sulfonic acid (TNBS). Pathologic histology changes and collagen fiber hyperplasia in the colon were observed under a light microscope by Hematoxylin and Eosin Staining and Masson Trichrome staining.The expression of insulin-like growth factor (IGF-1), transforming growth factor-β1(TGF-β1) and connective tissue growth factor (CTGF) in colon were measured by immunohistochemistry (IHC).Results:1The histology by hematoxylin and eosin staining shown that intestine structure and mucosa were normal in D group. There were damages in the colon mucosa of model rats with disorder structure and thickening bowel obviously. Pathologic histology was improved in Y group and J group.2.Comparing with D group, the collagen hyperplasia and expressions of IGF-1、TGF-β1and CTGF protein were significantly increased in M group. Collagen hyperplasia decreased significantly in Y group and J group compared with M group. The effect in J group was better than that in Y group. The expression of IGF-1, TGF-β1and CTGF protein decreased significantly in Y group and J group. The expression of IGF-1, TGF-β1protein decreased significantly in J group than that in Y group. The expression of CTGF protein was no obvious difference between Y group and J group.Conclusions:The modified Kuikeling have the effects on anti-fibrosis in colitis model rats. The mechanisms may be due to reducing collagen deposition and down-regulating the expression of IGF-1, TGF-β1and CTGF in colon. |
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