Effects Of Berberine On Inflammatory Factors,Adipokines And Metabolism Of Fatty Acid In3T3-L1Adipocytes

Objectives:1. To investigate the effects of berberine on the mRNA expression of tumor necrosis factor-α and interleukin-6in3T3-L1adipocytes.2. To investigate the effects of berberine on the mRNA expression of leptin, adiponectin and visfatin in3T3-L1adipocytes.3. To investigate the effects of berberine on the mRNA expression of fatty-acid synthase, acetyl-CoA carboxylase, adipose triglyceride lipase and adipocyte fatty acid binding protein in3T3-L1adipocytes.Materials and methods:Differentiated3T3-L1adipocytes were grouped as following:1. berberine dosing groups:blank group,0μmol/L group (control group),5μmol/L group,10μmol/L group,20μmol/L group and40μmol/L group. All the groups were treated by berberine of different dose for24hours.2. berberine timing groups:blank group, control group, and10μmol/L group treated by berbrine for4,8,12and24hours patternly. Total RNA of adipocytes was extracted after the berbrine treatment as above. The mRNA expression of inflammatory factors (TNF-α and IL-6), adipokines (leptin, adiponectin and visfatin) and fatty acid metabolism-related lipase and protein (FAS, ACC, ATGL and AFABP) was detected by quantitative real time polymerase chain reaction (qRT-PCR).Results:1. Effects of Berbrine on mRNA expression of TNF-α and IL-6There were significant differences in TNF-α mRNA expression among BBR dosing groups (F=61.819, P<0.001). Berberine lowered mRNA expression of TNF-α in dose-dependent manner, while its dose was within5-20μmol/L. There were significant differences in TNF-α mRNA expression among BBR timing groups (F=106.767, P<0.001).Berberine lowered mRNA expression of TNF-α in time-dependent manner. There were significant differences in IL-6mRNA expression among BBR dosing groups (F=111.505, P<0.001). Berberine lowered mRNA expression of IL-6in dose-dependent manner, while its dose was between5-20μmol/L. There were significant differences in IL-6mRNA expression among BBR timing groups (F=65.528, P<0.001). Berberine lowered mRNA expression of IL-6in time-dependent manner.2. Effect of BBR on mRNA expression of leptin, adiponectin and visfatinThere were significant differences in leptin mRNA expression among BBR dosing groups (F=181.846, P<0.001). Berberine lowered mRNA level of leptin in dose-dependent manner, while its dose was between5-20μmol/L. There were significant differences in leptin mRNA exression among BBR timing groups (F=39.065, P<0.001). Berberine lowered mRNA expressionk of leptin in time-dependent manner.There were no significant differences in adiponectin mRNA expression among BBR dosing groups (P>0.05). There were significant differences in adiponectin mRNA expression among BBR timing groups (F=4.323, P=0.018).Berberine lowered mRNA expression of adiponectin after24-hours treatment at the dose of10μmol/L.There were significant differences in visfatin mRNA expression among BBR dosing groups (F=170.723, P<0.001). Berberine increased visfatin mRNA expression in dose-dependent manner, while its dose was between10-20μmol/L. There were significant differences in visfatin mRNA expression among BBR timing groups (F=24.451, P<0.001).Berberine increased mRNA expression of visfatin in time-dependent manner.3. Effect of BBR on mRNA expression of FAS, ACC, ATGL and AFABPThere were significant differences in FAS mRNA expression among BBR dosing groups (F=73.369, P<0.001). Berberine lowered mRNA expression of FAS in dose-dependent manner, while its dose was between5-20μmol/L. There were significant differences in m FAS mRNA expression among BBR timing groups (F=78.562, P<01.001). Berberine lowered mRNA expression of FAS significantly, while its time was between the24to48hours.There were no significant differences in mRNA expression of ACC among BBR dosing groups (P>0.05). So were the BBR timing groups.There were significant differences in mRNA expression of ATGL among BBR dosing groups (F=14.834, P<0.001). Berberine lowered mRNA expression of ATGL in dose-dependent manner, while its dose was between5-20μmol/L. There were significant differences in mRNA expression of ATGL among BBR timing groups (F=20.761, P<0.001). Berberine lowered mRNA expression of ATGL significantly, while its time was between8to48hours.There were significant differences in AFABP mRNAexpression among BBR dosing groups (F=11.693, P<0.001). Berberine lowered mRNA expression of AFABP in dose-dependent manner, while its dose was between10-20μmol/L. There were significant differences in AFABP mRNA expression among BBR timing groups (F=13.096, P<0.001). Berberine lowered mRNA expression of AFABP significantly after4hours-treatment.Conclusions:1. Berberine could improve the inflammation of3T3-L1adipocytes within a certain range of dose or time.2. Berberine could regulate the adipokines of3T3-L1adipocytes within a certain range of dose or time.3. Berberine could inhibit the generation of fatty acid and hydrolysis of triglyceride of3T3-L1adipocytes within a certain range of dose or time.4. Berberine could improve insulin resistance through regulating the inflammation, adipokines and fatty acid metabolism.