The Diagnose Of Human Tissue Based On Multiphoton Microscopy

Multiphoton Microscopy (MPM) imaging based on two-photon excited fluorescence (TPEF) and Second-harmonic generation (SHG) allows simultaneous visualization of cellular species and extracellular matrix components. It has become a strong tool in noninvasive diagnoses of human tissue because it can provide enhanced imaging penetration depth, reduced out-of-focus photobleaching and phototoxicity, which makes it more suitable to study live animals. In this thesis, human skin dermis and submucosa of colon and stomach tissues were investigated based on MPM. By system analys, the different components of the microscopic structure of collagen and elastic fibers in different human tissue will be obtained. The features such as the appearance of abnormal cells and the large loss of collagen in cancerous human tissue were extracted to be as significant indicators to distinguish cancerous human tissue from normal human tissue. Our results show that MPM based on TPEF and SHG has great value to be applied in vivo real-time diagnosis and therapy in diseases of human tissues.