New Method Of Making Animal Model Of Limb Ischemia Reperfusion Injury

Objective: Pneumatic tourniquet was used to make limb ischemia reperfusion ofanimal model to investigate the injury of peripheral nerve and the skeletal muscle.Methods: Choose the thirty health6-month-old New Zealand white rabbits (weighing3.5±0.3Kg), male or female, randomly divided into3groups of10each, group A: acontrol group B: ischemia2hours, group C: ischemia4hours. The left hind limb inrabbits circled with inflatable tourniquet release at different times, resulting in the lefthind limb ischemia-reperfusion injury model. Except group A all the groups were circledwith inflatable tourniquet. Each group were measured indicators of limbelectrophysiology reperfusion after1hour,2hours,3hours,4hours,5hours,6hours.The muscle of group A was observed by morphology after6hours. The muscle of groupB and C were observed by morphology6hours after reperfusion. The walking functionof left hind limb of every rabbits was evaluated5days after surgery. Results: With thelonging of ischemia-reperfusion time, compare group A with group B and C we can findthat, the incubation period of the peripheral nerves extend, lower amplitude andconduction velocity decreased, the incubation, amplitude and conduction velocitybetween three groups have statistically significant difference (P＜0.05). We observed theskeletal muscle with microscopy (group B and C), stripes disorders, muscle fiber rupture,interstitial vascular dilatation and congestion, a large number of neutrophils infiltration.Conclusion: After the interaction of ischemia and reperfusion the physical damage andfurther increase the functionality, or even irreversible lesions. The animal model makingis less damage to animals, and approach to the clinic more.