| Objective: To observe the effects of BMSCs transplantation on vascularizationduring hepatocarcinogenesis in rats. Methods:1. We choose30female Wistar rats andtake the random touch ball method divided them into BMSCs transplantation group (10rats) and pure molding group (10rats) and empty control group (10rat). The first twogroups were accepted diethylnitrosamine to develop liver cancer. BMSCs transplantationgroup was injected BMSCs in different time point during molding process. Pure moldinggroup were injected equal normal saline for control. The blank control group was onlyaccepted normal water as control.2. The rats were breed in same condition for21weeksand observed the following index: a. By MRI, to observe the difference of liver of threegroup; b. By the H-E staining, to observe the pathomorphological change of liver of threegroup. c. Detect existence of SrY by hybridization in situ, to confirm successfultransplantation of BMSCs. d. Detect the level of expression of MVD and VEGF by theimmunohis-tochemistry, to observe difference of three group. Results:1. By HE stainingconfirmed eight rats in the pure model group and nine rats in the BMSCs transplantationgroup were proved to be with liver cancer;2. The SrY+cells were seen in the BMSCstransplantation group;3. MRI showed that there were more space-occupying lesions inthe liver of the BMSCs transplantation group compared with the pure model group(t=2.45，P=0.026);4. The average positive expression rate of vascular endothelialgrowth factor and microvessel density in the BMSCs transplantation group were higherthan that in the pure model group (P<0.05). Conclusion: In the hepatoma models of ratsprepared with diethylnitrosamine, BMSCs can promote the vascularization resulting inthe development of liver cancer. |
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